PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H11 AGAINST GASTRIC CANCER CELL

The object of this study is to investigate the properties of gastric cancer assoiciated antigen by McAb3H11 against gastric cancer cell line. Antigen purified by affinity chromatography and further characterized by biochemical and immunological techiques. Results showed that assoiciated antigen of McAb 3H11 was mainly located on the membrane of target calls. It was sensitive to heat and digestion of proteases,but resistant to treatment with sodium perioclate. Schiff‘s reagent staining was negative. SDS-PAGE and IEF-analysis showed that the antigen was a 210 kD molecular weight with pl of 8.5 protein. The molecular weight of antigen extracted from target cell with tunicamycin was not changed. We concluded that the corresponding antigen of MeAb 3H11 might be an alkaline protein.

PURIFICATION AND IDENTIFICATION OF THE ANTIGEN ASSOCIATED WITH MONOCLONAL ANTIBODY 3H_(11) AGAINST GASTRIC CANCER CELL

The object of this study is to investigate the properties of gastric cancer assoiciated antigen by McAb3H_(11) against gastric cancer cell line. Antigen purified by affinity chromatography and further charac- terized by biochemical and immunological techiques. Results showed that assoiciated antigen of McAb 3H_(11) was mainly located on the membrane of target cells. It was sensitive to heat and digestion of proteases,but resistant to treatment with sodium periodate. Schiff's reagent staining was negative. SDS-PAGE and IEF- analysis showed that the antigen was a 210 kD molecular weight with pl of 8. 5 protein. The molecular weight of antigen extracted from target cell with tunicamycin was not changed. We concluded that the cor- responding antigen of McAb 3H_(11) might be an alkaline protein.