| 泵抑制剂对环丙沙星在铜绿假单胞菌内聚积的影响 |
| |
| 引用本文： | 赵廷坤,毛淑梅,史立宏,房春燕. 泵抑制剂对环丙沙星在铜绿假单胞菌内聚积的影响[J]. 中国实用医药, 2008, 3(27): 35-36 |
| |
| 作者姓名： | 赵廷坤 毛淑梅 史立宏 房春燕 |
| |
| 作者单位： | 应用药理学实验室,潍坊医学院药理学教研室,261042 |
| |
| 摘 要： | 目的探讨耐药铜绿假单胞菌（PA）中是否存在主动外排系统,以揭示PA对氟喹诺酮类药物的耐药机制。方法检测碳酰氰间氯苯腙（CCCP）对16株PA药物M IC的影响;荧光法检测PA耐药菌和敏感菌对环丙沙星（CIP）的摄取,以及加入CCCP后对CIP累积量的变化情况。结果外排泵阳性株为4株,CCCP可以使PA 16菌体内环丙沙星的累积量显著上升,而其它菌株中加入CCCP后,环丙沙星的累积量变化不大。结论本地区PA中可能存在氟喹诺酮药物的主动外排系统。
|
| 关 键 词： | 铜绿假单胞菌 氟喹诺酮药物 主动外排系统 |
Effects of pump inhibitor on accumulation of ciprofloxacin in pseudomonas aeruginosa clinical isolates |
| |
| ZHAO Ting-kun,MAO Shu-mei,SHI Li-hong,et al.. Effects of pump inhibitor on accumulation of ciprofloxacin in pseudomonas aeruginosa clinical isolates[J]. China Practical Medical, 2008, 3(27): 35-36 |
| |
| Authors: | ZHAO Ting-kun MAO Shu-mei SHI Li-hong et al. |
| |
| Affiliation: | ZHAO Ting-kun,MAO Shu-mei,SHI Li-hong,et al.Department of Pharmacology,Applied Pharmacological Laboratory,Weifang Medical University,Weifang 261042,Shandong Province,China |
| |
| Abstract: | Objective To explore whether active efflux system is present in P. aeruginosa in order to identify the resistance mechanism of P. aeruginosa to Fluoroquinolones antibiotics. Methods The influence of carbonyl cyanide-m-chlorophenylhydrazone （CCCP） on MICs of antibiotics to P. aeruginosa clinical isolates were tested by agar dilution according to NCCLS. The accumulation of ciprofloxacin in P. aeruginosa were measured by a fluorescence method and the influence of CCCP was analyzed. Results 4 P. aeruginosa isolates had the efflux pumps for β-lactam antibiotics or fluoroquinolones. CCCP could dramatically increase the accumulation of ciprofloxacin in P. aeruginosa PA16. However, CCCP had little effect on the accumulation of ciprofloxacin in other P. aeruginosa clinical isolates. Conclusion There may have an active efflux system targeting fluoroquinolones in P. aeruginosa. |
| |
| Keywords: | P.aeruginosa Fluoroquinolones Active efflux system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
