胆红素水溶液测定方法研究

使胆红素生成钠盐后,加入适当的抗氧剂和金属离子络合剂,使胆红素钠盐稳定,用分光光度法进行含量测定。本法克服了重氮化法和摩尔吸收系数法操作烦琐、有机镕剂昂贵和污染等弊端,获得操作简便、准确、快速的满意结果。

Study on the New Method of Determination of Aqueous Solution of Bilirubin

This paper deseribed the new method of determination of aqueous solution of bilirubin. Afert bilirubin was converted into its Na salt in aqueous solution, antioxident and complex-cation agent were added, the Na salt was stable and could be determined by spectrophotometry. The method not only overcame the difficult operations of the determination by azide and molar absorptivity, and the high cost of the organic solvents and pollutions, but also simplified the operations and made it accurate and rapid. The satisfactory results were obtained after mathematical statistics analysis.