冻干血小板再水化后聚集

本研究旨在观察冻干血小板再水化后聚集反应性变化。以4种不同浓度的凝血酶、瑞斯托霉素、ADP、胶原作诱导剂,使用APACT2型血小板聚集仪,检测冻干再水化和新鲜血小板的最大聚集率,以ADP为诱导剂,检测胞内外海藻糖对再水化冻干血小板最大聚集率的影响。结果表明当凝血酶、瑞斯托霉素、ADP、胶原浓度分别为1U/ml、1.6mg/ml、20μmol/L、2μg/ml时,新鲜血小板最大聚集率达100%左右,冻干再水化血小板最大聚集率分别达(70.17±7.36)%、(15.3±2.81)%、(68.67±6.86)%、(64.67±11.6)%。胞内外海藻糖组、胞外海藻糖组、空白对照组冻干再水化血小板最大聚集率分别达(66.0±4.69)%、(25.3±2.42)%、(11.5±1.87)%(P<0.01)。结论凝血酶、瑞斯托霉素、ADP、胶原浓度分别为1U/ml、1.6mg/ml、20μmol/L、2μg/ml可作为血小板聚集实验的合适浓度,胞内外海藻糖使冻干再水化血小板最大聚集率显著提高。

Aggregation after Rehydration of Lyophilized Platelets

This study was aimed to investigate the aggregation of rehydrated-lyophilized platelets. The aggregation rate of fresh and rehydrated-lyophilized platelets were measured by using thrombin, ristocetin, ADP and collagen as inductors and APACT2 aggregameter; the effects of intra- and extra-cellular trehalose on maximum aggregation rate of rehydrated-lyophilized platelets were detected by using ADP as an inductor. The results showed that the aggregation rate of fresh platelets was all about 100%, while aggregation rate of rehydrated lyophilized platelets was (70.17 +/- 7.36)%, (15.3 +/- 2.81)%, (68.67 +/- 6.86)%, (64.67 +/- 11.6)% respectively, when the concentration of thrombin, ristocetin, ADP and collagen was 1 U/ml, 1.6 mg/ml, 20 micromol/L and 2 microg/ml. The maximum aggregation rates of rehydrated-lyophilized platelets in intra- and extra-cellular trehalose, extracellular trehalose and blank control groups were (66.0 +/- 4.69)%, (25.3 +/- 2.42)% and (11.5 +/- 1.87)% (P < 0.01), meanwhile there was significant difference of rehydrated-lyophilized platelet aggregation rate between intra- and extra-cellular trehalose and extracellular trehalose groups (P < 0.01). It is concluded that the concentrations of thrombin (1 U/ml), ristocetin (1.6 mg/ml), ADP (20 micromol/L) and collagen (2 microg/ml) are optimal for platelets aggregation tests, the internal and extracellular trehalose significantly enhance the aggregation of rehydrated-lyophilized platelets.