亚硒酸钠对顺铂致人体淋巴细胞增殖抑制的拮抗作用

目的 检测亚硒酸钠是否能够削弱或解除顺铂对植物血球凝激素(PHA)刺激的人体外周血淋巴细胞的增殖抑制。方法 用顺铂和亚硒酸钠单独或联合处理PHA刺激的人体外周血淋巴细胞，顺铂(0．05，0．20，0．50mg／L)在细胞培养24h时加入，亚硒酸钠(0．05mg／L)在不同处理中分别在细胞培养开始时加入或与顺铂同时加入；培养72h后检测转化淋巴细胞的有丝分裂指数。结果0．05mg/L亚硒酸钠在培养开始时加入，PHA刺激转化的淋巴细胞有丝分裂指数较对照增长42．8％(P＜0．05)，与顺铂同时加入，有丝分裂指数增长13．7％(P＞0．05)。0．05与0．20mg/L顺铂处理细胞，有丝分裂指数未发生显性改变，当顺铂剂量为0．50mg/L时，细胞有丝分裂指数较对照降低54．5％(P＜0．001)。亚硒酸钠预处理细胞可以解除0．50mg/L顺铂所致的细胞增殖抑制，使有丝分裂指数恢复正常，但亚硒酸钠与顺铂同时加入时，被顺铂抑制的有丝分裂指数只能部分提高。结论0．05mg/L亚硒酸钠单独作用于淋巴细胞可直接促进细胞增殖，与顺铂联合处理细胞时，可降低顺铂毒性，拮抗顺铂的抗增殖作用。亚硒酸钠在细胞培养开始时加入培养体系效果更佳。

Antagonism of sodium selenite on inhibition of human lymphocyte proliferation induced by cispatin

Objective To examine whether sodium selenite could weaken or remove the proliferative inhibition of phytohaemagglutinin(PHA)-stimulated human peripheral blood lymphocytes(PBL) induced by cispatin.Methods PHA-stimulatd human PBL were incubated with cisplatin alone or in combination with sodium selenite.Cisplatin was administered to the 72 hours cultures of PBL with three different concentration(0.05,0.20,0.50?mg/L) at 24 hours after PHA stimulation.Sodium selenite was added with a single dose of 0.05?mg/L at 0 hour or at 24 hours after PHA stimulation for different treatment.Mitotic indices(MI) of transformed lymphocytes were tested at the end of 72 hours cultures.Results Sodium selenite at dose of 0.05mg/L enhanced MI of transformed lymphocytes in response to PHA stimulation by 42.8%(P<0.05) and 13.7%(P>0.05) above the control values when the chemical was administered respectively at 0 hour and at 24 hours after PHA stimulation.When cells were exposed to cisplatin from 0.05mg/L to 0.20mg/L,Mi of transformed lymphocytes had no significant alteration compared with control cells,however,when exposed to cisplatin at 0.50mg/L,MI was reduced by 54.5%(P<0.001) compared to control cells.Proliferative inhibition caused by cisplatin at 0.50mg/L were counteracted and a normal MI was obtained when the cells were pretreated with sodium selenite,but when sodium selenite was simultaneously added with cisplatin,the MI inhibition by the agent could only partially enhanced.Conclusion Sodium selenite at 0.05 mg/L could directly promote cell proliferation when PBL were treated with it alone.When it was used as protectant to cotreat PBL in combination with cisplatin,it can reduce cisplatin cytotoxicity,and protect PBL against the antiproliferative effect of the agent.More effective results could be obtained when it was administered to the system at the beginning of the culture.