急性髓系白血病亚型5’-核苷酸酶超微结构分析

5’-核苷酸酶（5’NT）为嘌呤代谢酶，能水解核苷酸为核苷与磷酸，兼有磷酸转移酶活性，对维持核苷酸代谢平衡有重要作用。本研究主要探讨急性髓系白血病不同亚型患者及正常人粒细胞5’NT膜表达特点。用白细胞分离液分离33例急性粒细胞白血病患者和5例正常人骨髓有核细胞，以胞苷-5’-单磷酸（CMP）为底物进行电子显微镜和细胞组织化学染色，在观察细胞结构基础上分析粒细胞阳性率和阳性指数。结果表明，阳性反应产物主要分布于粒细胞膜，正常人大部分粒细胞呈阴性反_应，或反应很弱；急性髓系白血病M0、M1、M2和t（8；21）各型粒细胞阳性率和阳性指数升高，但M。、M．＋M2和t（8；21）三组之间差别不明显。t（15；17）细胞阳性率和阳性指数低于其它亚型；与正常人相比无统计学差别。结论：5’NT表达强弱可能与急性白血病粒细胞分化程度有关，t（15；17）白血病可能属于高分化性髓系向血病。

Ultrastructural Analysis of 5" Nucleotides Distribution in Acute Myeloid Leukemia Subtypes

5' nucleotides (5'NT), a purine degradative enzyme, is capable of hydrolyzing nucleotide and acting as a phosphotransferase simultaneously. It has critical role in maintaining nucleotide metabolism balance. The present study was aimed to investigate the expression of 5'NT in bone marrow granulocytes (BMGs) from patients with acute myeloid leukemia (AML) and healthy donors comparatively. The BMGs were isolated from bone marrow of 33 patients with AML and 6 healthy donors by using lymphocyte isolating solution. The reactivity of 5'NT was detected by electron microscope and cytochemistry of cytidine monophosphate (CMP). The positive BMG ratio and their index were calculated on the base of ultrastructural observation semiquantitatively. The results indicated that electron microscopy revealed plasma membrane reacting pattern of CMP. Most BMGs from normal donors were CMP negative or exhibited lower active degree. All cases of M(0), M(1), M(2) and t (8; 21) showed high positive percentages and high indexes of BMGs, but no statistic differences between them. APL of t (15; 17) shared lower percentages and indexes than other subtypes. There was no significant difference between APL and normal donors statistically. In conclusions, the results suggested the expression of 5'NT may be associated with BMG differentiation in AML, and APL of t (15; 17) may be a highly differentiated leukemia subtype.