血瘀证患者单核细胞和中性粒细胞趋化游走能力变化及益气活血中药干预作用

[目的]观察血瘀证患者外周血单核细胞 (PBMNC)和中性粒细胞 (Neu)趋化游走能力变化及益气活血中药 (黄芪丹参颗粒 )干预作用 ,明确血瘀证发病机制及中药疗效机制。[方法]观察益气活血中药治疗前后血瘀证患者PBMNC和Neu跨越聚碳酸酯膜 (PC)和血管内皮细胞 (HUVEC)单层的个数。[结果]血瘀证患者PBMNC趋化游走能力显著高于健康者 ,益气活血中药治疗后 ,血瘀证患者PBMNC和Neu趋化游走能力显著降低。[结论]提示炎性反应参与血瘀证的形成 ,而益气活血中药具有通过降低炎性反应缓解血瘀证的作用机制

Chemotaxis and migration changes of monocyte and neutrophil in cases with blood stasis and interference effect of Chinese materia medica which can tonifying qi and promoting blood circulation

To observe the chemotaxis and migration changes of peripheral blood monocyte (PBMNC) and neutrophil (NEU) in cases with blood stasis and interference effect of Chinese materia medica (Huangqi Danshen Granule),which can tonify qi and promote blood circulation. To study the mechanism of the attacking of blood stasis and the effect of Chinese materia madica.After treating with Huangqi Danshen Granule, the PBMNC and NEU, migrated across polycarbonate membrane and manoendotheliocyte layer were accounted.The chemotactic and migrating ability changes of PBMNC and NEU in cases with blood stasis lowered obviously.[Conclusion] The blood stasis may be associated with inflammatory reaction. The Chinese materia medica can relieve the blood stasis by lowering the inflammation reaction.