中药成分BP对老年人淋巴细胞凋亡的调控作用

目的 :研究老年人淋巴细胞亚群及其细胞凋亡过程 ,探讨中药成分对细胞凋亡的调控作用。方法 :用间接免疫荧光技术检测淋巴细胞表型 ,用MTT法测定淋巴细胞增殖应答 ;用流式细胞仪及自动图像分析来检测细胞凋亡。结果 :老年人淋巴细胞比青年人淋巴细胞的增殖应答能力低 ,CD4 5RA 细胞亚群减少 ,而CD4 5RO 细胞亚群增多 ,后者易发生凋亡。而中药成分BP对老年人淋巴细胞凋亡有抑制作用。结论 :老年人淋巴细胞易感细胞凋亡 ,取决于活化 ,称为活化诱导性细胞死亡 ,此在免疫衰老中起着重要作用提示可以从中药开发细胞凋亡的调节药物。

The modulatory effect of Chinese herb substances BP on apoptosis of lymphocytes in the elderly

Objective:To study the changes of lymphocytes subpopulation and apoptosis process of lymphocytes in the elderly and to investigate the modulatory effect of BP from Chinese herb on apoptosis.Methods:Lymphocytes phenotype were determinated using indirect immunofluorescence technique.The proliferative responses were measured with MTT method.Apoptosis of lymphocytes was evaluated by flow cytometry and automatic image analysis.Results:The proliferative responses of lymphocytes in the elderly were lower than that of young adults.Decreased CD45RA positive cells and increased CD 45 RO positive cells were found in lymphocytes population of aged people compared to that of young adults. The CD 45 PO positive cells were prone to apoptosis.There is an imhibitory effect of BP on apoptosis of lymphocytes in the elderly.Conclusion:It was implicated that the susceptibility of lymphocyte in the elderly to apoptosis depends on activation,so called activation induced cell death.Present results suggested that apoptosis of lymphocytes of aged people play an important role in the pathogenesis of immunosenescence.The possibility appeared for development of modulatory drugs on apoptosis from Chinese herbs.