液相色谱-电喷雾离子阱质谱法分析大鼠血浆中的樟柳碱及其代谢物

目的用液相色谱-电喷雾离子阱串联质谱(LC-MS^{n)联用方法鉴定大鼠血浆中的樟柳碱及其主要代谢物。方法取单剂量灌胃樟柳碱20 mg的大鼠血浆，甲醇沉淀蛋白，采用LC-MSn等方法分析血样。与空白血样及樟柳碱对照品相比较，根据血样中代谢物相对分子质量的变化及其多级质谱数据，鉴定并阐述其结构。结果在服药后的大鼠血样中发现4种代谢物， 分别为东莨菪醇、 N-去甲基东莨菪醇、 羟基化樟柳碱以及N-氧化樟柳碱。结论 该方法灵敏、快速、简便，适合于药物及其代谢物的快速鉴定。}

Analysis of anisodine and its metabolites in rat plasma by liquid chromatography-tandem mass spectrometry

Aim To identify anisodine and its metabolites in rat plasma after ingestion of anisodine by combining liquid chromatography and tandem mass spectrometry (LC-MS~n). Methods Plasma sampls from rats after a single orally administration of 20 mg anisodine were added with methanol to precipitate protein. Then, it was analyzed by LC-MS~n. Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses, retention-times and full scan MS~n spectra with those of the parent drug and blank plasma. Results The results revealed that the parent drug and its four metabolites (norscopine, scopine, hydroxyanisodine, N-oxide anisodine) existed in rat plasma. Conclusion This method is sensitive, rapid, simple, and it is suitable for the rapid identification of drug and its metabolits.