酵母菌发酵黄芪的抑菌作用初探

目的:研究黄芪酵母菌发酵液的体外、体内抑菌活性。方法:体外实验采用滤纸片扩散法,通过测定抑菌圈直径考察黄芪发酵液对金黄色葡萄球菌、大肠杆菌、肺炎链球菌、绿脓杆菌、普通变形杆菌的体外抑菌活性;采用2倍稀释法检测黄芪发酵液对各致病菌的最小抑菌浓度(MIC);体内实验以金黄色葡萄球菌为指示菌种,观察黄芪酵母菌发酵液不同浓度(0.1 g/mL,1.0 g/mL,2.5 g/mL)灌胃给药7 d对感染小鼠的死亡率。结果:体外实验结果显示:黄芪发酵液对5种致病菌均有一定抑制作用,黄芪发酵液高浓度组对致病菌具有较强的抑制作用;对金黄色葡萄球菌、大肠杆菌、肺炎链球菌、绿脓杆菌、普通变形杆菌的最小抑菌浓度分别为0.313 g/mL,0.078 g/mL,0.625 g/mL,0.313 g/mL,0.156 g/mL;体内实验研究表明:黄芪发酵液低、中、高3个浓度组均能显著降低金黄色葡萄球菌引起的小鼠死亡率,死亡率分别是65%,45%,30%,与细菌对照组死亡率90%比较,差异具有统计学意义(P0.05,P0.01)。结论:黄芪酵母菌发酵液体外、体内对受试菌均有一定抑制作用。

Study on bacteriostasis of Huangqi fermentation broth

Objective：To study the bacteriostasis of Huangqi fermentation broth in vitro and in vivo. Method：Inhibition zone diameters were measured to investigate bacteriostasis of Huangqi fermentation broth against staphylococcus aureus,bacillus coli, streptococcus pneumoniae, pseudomonas aeruginosa and proteus vulgaris in vitro. Minimal inhibitory concentrations（MIC） against the above pathogens were determined by double dilution method. Bacteriostasis in vivo was determined in the mice model with peritoneal injection of staphylococcus aureus. Results：MIC of Huangqi fermentation broth against staphylococcus aureus,bacillus coli,streptococcus pneumoniae,pseudomonas aeruginosa and proteus vulgaris were 0.313 g/mL,0.078 g/mL, 0.625 g/mL,O.313 g/mL, 0.156 g/mL, respectively. The mortality rates of mice in Huangqi fermentation broth low, middle, high dosage group were 65% ,45%, 30%,respectively. Compared with control group, mortality rate of Huangqi fermentation broth groups was decreased（P〈0.05,P〈0.01）. Conclusion：Huangqi fermentation broth has bacteriostatic activity on tested bacterias in vitro and in vivo.