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| 反相高效液相色谱法测定解毒消银丸中丹酚酸B的含量 | |
| 引用本文： | 刘贵昌,段秀俊,李慧峰,阴孟凡. 反相高效液相色谱法测定解毒消银丸中丹酚酸B的含量[J]. 山西中医学院学报, 2014, 15(4): 42-44 |
| 作者姓名： | 刘贵昌 段秀俊 李慧峰 阴孟凡 |
| 作者单位： | 1. 山西省儿童医院妇幼保健院,山西太原,030013 2. 山西中医学院,山西太原,030024 3. 山西中医学院白癜风研究所,山西太原,030002 |
| 摘 要： | 目的:建立解毒消银丸中丹酚酸B含量的RP-HPLC测定方法。方法:采用反相高效液相色谱法,C18色谱柱(4.6mm×250 mm,5μm);流动相:甲醇∶乙腈(3∶1)-1.0%甲酸水溶液(34∶66),流速:1 mL/min;柱温:30℃;检测波长:286 nm。结果:丹酚酸B在220 ng~4 440 ng范围内与峰面积呈良好线性,r=0.999 6,平均回收率为99.79%,RSD为1.79%。结论:该方法重现性好,准确度高,可用于解毒消银丸的质量控制。 |
| 关 键 词： | 解毒消银丸 反相高效液相色谱法 丹酚酸B |
Determination of salvianolic acid B content in Jiedu Xiaoyin pills by RP-HPLC |
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| Liu Guichang,Duan Xiujun,Li Huifeng,Yin Mengfan. Determination of salvianolic acid B content in Jiedu Xiaoyin pills by RP-HPLC[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2014, 15(4): 42-44 | |
| Authors: | Liu Guichang Duan Xiujun Li Huifeng Yin Mengfan |
| Affiliation: | liu Guichang,Duan Xiujun,Li Huifeng,Yin Mengfan( LMaternal and Child Health Hospital,Shanxi Province Children＇s Hospital,Taiyuan Shanxi 030013;2.Shanxi College of TCM, Taiyuan Shanxi 030024 ; 3. Vitiligo Institute, Shanxi College of TCM, Taiyuan Shanxi 030002) |
| Abstract: | Objective：To establish a method for determinating salvianolic acid B content in Jiedu Xiaoyin pills. Methods： RP-HPLC was performed on Cls column （4.6 mm × 250 mm,5 μm） with methanol：acetonitrile （3：1） - 1% aqueous formic acid （34：66） as mobile phase. Flow rate was 1 mL/min. Column temperature was 30 ℃ and the detection wavelength was 286 nm. Results：Salvianolic acid B had a good linear from 220 ng to 440 ng,r=0.999 6. The average recovery rate was 99.79% and RSD wasl.79%. Conclusion ：This method has good reproducibility, high accuracy, and could control quality of Jiedu Xiaoyin pills. |
| Keywords: | Jiedu Xiaoyin pills RP-HPLC salvianolic acid B |
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