清肠栓对大鼠溃疡性结肠炎急性期模型CINC-1的影响 |
| |
引用本文: | 施斌,谢建群,袁建业,张涛,郑昱,马贵同.清肠栓对大鼠溃疡性结肠炎急性期模型CINC-1的影响[J].中国中医急症,2014(6):1005-1006,1011. |
| |
作者姓名: | 施斌 谢建群 袁建业 张涛 郑昱 马贵同 |
| |
作者单位: | [1]浙江中医药大学第二门诊部,浙江杭州310009 [2]上海中医药大学附属龙华医院上海中医药大学脾胃病研究所,上海201203 [3]广西中医药大学,广西南宁530000 |
| |
基金项目: | 国家自然科学基金资助项目(No.30772801) |
| |
摘 要: | 目的观察清肠栓对大鼠溃疡性结肠炎(UC)急性期模型结肠黏膜CINC-1蛋白含量的影响。方法SD大鼠48只,分为正常组、模型组、清肠栓组和SASP组,每组12只。用三硝基苯磺酸(TNBs)诱导大鼠UC急性期模型。造模后第3日开始给药,连续给药7 d后处死。ELISA检测病变部位结肠组织中的CINC-1蛋白含量。结果 CINC-1蛋白含量,模型组较正常组明显升高(P0.05),清肠栓组及SASP组较模型组均降低(P0.05)。结论清肠栓通过调节结肠黏膜CINC-1蛋白含量,减少中性粒细胞向病变局部黏膜组织的趋化和激活,从而起到缓解病变部位炎症的作用。
|
关 键 词: | 清肠栓 溃疡性结肠炎 中性粒细胞趋化因子-1 |
Effect of Qingchang Suppository on CINC-1 in an Experimental Rat Model of Acute Ulcerative Colitis |
| |
Institution: | SHI Bin,XIE Jian-qun YUAN Jian-ye,et al.( 1.No.2 Outpatient Department of Zhejiang Chinese Medical University ,Zhejiang,Hangzhou 310009, China) |
| |
Abstract: | Objective:To observe the effect of Qingchang Suppository (QS) on CINC-1 in colon mucosa of an experimental rat model of acute ulcerative colitis.Methods:48 SD rats were randomly divided into 4 equal groups,twelve animals in each group such as:normal control,blank,QS solution,SASP solution.Rats model of ulcerative colitis were brought about by TNBs.Beginning from the third day,except for the normal group,each group was treated with an enema of 0.9% NACL,QS,SASP respectively.These rats were sacrificed 1 week later.Elisa was used to measure the content of CINC-1 in colon.Results:Compared to normal control,protein content of CINC-1 was increased in blank (P< 0.05),however QS solution and SASP solution are decreased compared to blank (P< 0.05).Conclusion:QS can reduce the infiltration and activation of polymorphnuclear neutrophil (PMN) by regulating the protein content of CINC-1 in colon mucosa,and then to cure acute ulcerative colitis. |
| |
Keywords: | Qingchang Suppository Ulcerative colitis Cytokine-induced Neutrophil Chemoattractant-1 (CINC-1) |
本文献已被 CNKI 维普 等数据库收录! |
|