染料木素对AD大鼠海马CaM-CREB信号转导通路相关蛋白的影响

目的:通过观察染料木素对Aβ25-35致阿尔茨海默病(AD)模型大鼠CaM-CREB信号转导通路相关蛋白的影响,探讨染料木素预防阿尔茨海默病的作用机制。方法:SD雌性老龄大鼠120只,随机分为空白组、模型组、染料木素高、中、低剂量组、雌激素阳性对照组,每组20只。预给药7 d后,采用β-淀粉样肽(Aβ)双侧海马注射建立阿尔茨海默病大鼠模型,继续给药21 d。采用Morris水迷宫实验观察大鼠学习记忆能力的变化,HE染色观察大鼠海马组织形态学变化,ELISA法检测大鼠海马钙调蛋白(CaM)含量,免疫组化SP法检测大鼠海马环腺苷酸反应元件结合蛋白(CREB)、磷酸化CREB的表达。结果:与模型组比较,染料木素各剂量组AD大鼠的学习记忆能力提高(P0.01),海马CaM含量、CREB和p-CREB的表达降低(P0.05)。结论:染料木素可能通过影响海马CaM-CREB信号转导通路相关蛋白表达起到预防AD的作用。

Effects of genistein on CaM-CREB signal transduction pathway related proteins in hippocampus of Alzheimer's disease rats

Objective：To investigate the effects of genistein on CaM-CREB signal transduction pathway related proteins in hippocampus of Alzheimer＇s disease （AD） rats and to observe the preventive mechanism of genistein on Alzheimer＇s disease. Method： 120 SD female old rats were randomly divided into blank group,model group,estrogen positive group,high, middle,low dosage of genistein group （n=20）. Alzheimer＇s disease rat models were established by injection of 13-amyloid peptide into two sides of hippocampus. The changes of acquisition and memory of rats were observed by morris water maze test and the change of morphology was assessed by HE staining. Content of CaM in hippocampus was detected by ELISA. The expressions of CREB and p-CREB were tested by immunohistochemistry SP method. Results：Compared with model group,abilities of acquisition and memory of AD rats were improved （P〈0.01）,while content of CaM, expressions of CREB and p-CREB in hippocampus were decreased in genistein groups （P〈0.05）. Conclusion ：The genistein can play preventive role on AD by regulating expression of proteins related to CaM-CREB signal transduction pathway.