芪夏消瘿合剂对SD大鼠实验性自身免疫性甲状腺炎的影响

目的 观察芪夏消瘿合剂对SD大鼠实验性自身免疫性甲状腺炎（EAT）的影响.方法 将30只SD大鼠随机分为3组：正常对照组、EAT组、中药治疗组.对照组饮用自来水,其他各组实验SD大鼠饮用配制的0.05％碘化钠水溶液,并且在高碘的基础上皮下注射完全弗氏佐剂、甲状腺球蛋白和不完全弗氏佐剂,建立EAT动物模型.中药治疗组在不同的时间灌胃给予芪夏消瘿合剂治疗,观察大鼠甲状腺自身抗体水平的变化,并取完整甲状腺组织进行HE染色,观察其形态变化.结果 （1）造模第8周末,检测SD大鼠甲状腺球蛋白（TgAb）和甲状腺过氧化物酶抗体（TPOAb）.EAT组和治疗组的TGAb和TPOAb水平与对照组相比较,差异均有统计学意义（P＜ 0.01）;EAT组和治疗组之间TGAb和TPOAb水平相当（P＞0.05,检验效能＞0.999）;（2）实验第12周末,EAT模型组SD大鼠TGAb和TPOAb水平明显高于正常对照组（P＜0.01）;中药治疗组虽未能使TGAb和TPOAb水平恢复正常,其值明显低于EAT组（P＜0.01）.结论 芪夏消瘿合剂能够改善EAT大鼠的免疫功能紊乱状况.

Influence of Qixiaxiaoying Mixture on the Experimental Autoimmune Thyroiditis

Objective： To investigate the influence of Qixiaxiaoying Mixture on the experimental autoimmune thyroiditis in SI） rats. Methods： 30 SD rats were randomly divided into three groups including the normal control,theexperimental autoimmune thyroiditis （EAT） group,and the Chinese medicine treatment group. Except the normal controls,two other groups of rats were raised with water containing 0.05% NaI. EAT models were set up by some drugs such as the complete Freund＇s adjuvant,the thyroglobulin and the incomplete Freund＇s adjuvant. Qixiaxiaoying Mixture were given at different time in order to observe the change of the level about thyroid autoantibodies in the period of experiment. Results：（1 ）After eight weeks of molding,in EAT group and Chinese medicine treatment group. The differences of the levels of TGAb and TPOAb were not significance between above groups （P〉 0.05）. But comparing with normal group,these differences were obviouse（P〈 0.01 ）. （2）In the end of twelfth week, the levels of TGAb and TPOAb in EAT group were higher than those in normal group（P〈 0.01 ）. Although, the Chinese medicine treatment group were failed to make TGAb and TPOAb level to return to normal, the levels of TGAb and TPOAb in Chinese medicine treatment group were lower than those in EAT group （P〈 0.01 ）. Condusion： Qixiaxiaoying Mixture can prevent and improve immune function disorder status in SD rats with autoimmune thyroiditis.