翡翠贻贝多糖的制备及体外对Hela肿瘤细胞生长的抑制作用

目的制备翡翠贻贝多糖（PVPS）,并对其体外抗肿瘤活性进行研究。方法采用热水提取,Sevage-酶联用法除蛋白分离得到翡翠贻贝粗多糖,再经DEAE-Sepharose和Sepharose CL-6B柱层析纯化得到PVPS。MTT法研究其体外抗Hela肿瘤细胞的活性。结果PVPS的得率为0.86%,PVPS在25mg/L、50mg/L、100mg/L浓度时对体外培养的Hela肿瘤细胞有不同程度抑制作用（P〈0.05或P〈0.01）。结论从翡翠贻贝中制备得到的PVPS具有体外抑制肿瘤细胞生长的作用。

The preparation of Perna viridis polysaccharide and it's inhibiting efect on the growth of Hela tumor cell in vitro

Objective To prepare the polysaccharide from Perna viridis and to investigate it’s anti-tumor activity in vitro. Methods The crude polysaccharide was extracted from Perna viridis with hot water and the protein was removed using Sevage-enzymic method. The Perna viridis polysaccharide（PVPS） was purified u-sing DEAE-Sepharose and Sepharose CL-6B column chromatography. The in vitro anti-tumor activity of PVPS was assessed using MTT method. Results The yield of PVPS was 0. 86% . The PVPS （at 50 mg/L,100 mg/L, 200 mg/L） had different degrees of inhibitory effects on Hela tumor cells in vitro （P 0. 05 or P 0. 01）. Conclusion The Perna viridis polysaccharide has inhibiting efect on the growth of tumor cell lines in vitro.