肝细胞癌中OY-TES-1基因mRNA的表达

 目的：检测OY-TES-1基因mRNA在肝细胞癌(HCC)中的表达水平。方法:建立实时定量PCR方法，检测56例HCC及37例配对癌旁组织中目的基因OY-TES-1及看家基因HPRT的表达，OY-TES -1mRNA的表达量以OY-TES-1/HPRT表示，应用SPSS13.0统计分析软件进行数据分析。结果:（1）经实时定量PCR检测，HCC中OY-TES-1mRNA阳性率为73.21%（41/56），癌旁组织阳性率为 64.86%（24/37），有17对HCC及配对癌旁组织均表达阳性，OY-TES-1mRNA在HCC及癌旁组织中 的阳性表达率差异无统计学意义（P＞0.05）；（2）HCC中OY-TES-1mRNA水平明显高于对应的 癌旁组织（P＜0.05），但与HCC患者的年龄、性别等临床资料无关。结论:OY-TES-1 mRNA在HCC中有较高的表达频率，其表达水平高于对应的癌旁组织，它有望作为用于HCC辅助诊断及免疫治疗的肿瘤抗原。OY-TES-1蛋白在HCC及癌旁组织中的表达及相关意义尚值得做进一步的研究。

OY-TES-1 Gene mRNA Expression in Human Hepatocellular Carcinonta Tisses

Objective：To investigate the expression level of OY-TES-1 mRNA in human hepatocellular carcinoma （HCC）tissues.Methods：To establish quantitative real- time polymerase chain reaction for quantitative expression of OY-TES-1 mRNA.Fifty -six specimens of human HCC tissues and thirty-seven specimens of adjacent HCC tissues were analyzed for quantitative expression of target gene OY-TES-1 and housekeeping gene HPRT. OY-TES-1/HPRT was used to evaluate the expression level of OY-TES-1 mRNA.Data were dealt with statistical analysis software SPSS 13.0.Results ：(1) The expressive frequency of OY-TES-1 in HCC was 73.21%（41/56）while 64.86% （24/37）in adjacent HCC tissues by quantitative real-time PCR.Meanwhile, 17 pairs show positive, which had no significant difference（P＞0.05）.(2) The expression level of OY-TES-1 mRNA in human HCC tissues was higher than that in adjacent HCC tissues（P＜0.05）.No relationship was found between the expression of OY-TES-1 genes and clinic pathological parameters （P＞0.05）.Conclusion：With higher expressive frequency and level in human HCC, OY-TES-1 is a potential target for antigen-specific immunotherapy and auxiliary diagnosis of HCC.Our interest will now focus on the expression of OY-TES-1 protein in HCC and its significance.