核仁纺锤体相关蛋白1在胆管癌组织中的表达及其临床意义

目的:探讨胆管癌组织中核仁纺锤体相关蛋白1(Nusap1)的表达及临床意义。方法:通过GEPIA数据库分析Nusap1mRNA在胆管癌组织及正常胆管组织中的表达情况;用免疫组化技术检测61例配对的胆管癌及癌旁组织中Nusap1蛋白表达水平,分析其与胆管癌患者临床参数、总生存期的关系。结果:GEPIA数据库分析显示,与正常组织比较,Nusap1mRNA在胆管癌组织中高表达(P<0.05);免疫组化结果显示,胆管癌组织中Nusap1的阳性表达率明显高于对应癌旁组织(47.5%vs.14.8%,P<0.01);且其表达水平与患者临床TNM分期(P=0.001)、淋巴结转移(P=0.011)及术后生存状态(P=0.010)明显有关;Kaplan-Meier生存分析显示,Nusap1蛋白阳性患者术后中位生存时间为23个月,阴性患者术后中位生存时间为45个月,前者术后生存时间明显短于后者(P=0.000)。结论:Nusap1在胆管癌中高表达,并与胆管癌进展和不良预后密切相关,有望成为胆管癌的预后指标和治疗的潜在靶点。

Expression of nucleolar spindle associated protein 1 in cholangiocarcinoma tissue and its clinical significance

Objective: To investigate the expression of nucleolar spindle associated protein 1 (Nusap1) in cholangiocarcinoma tissue and its clinical significance.    Methods: The expressions of Nusap1 mRNA in cholangiocarcinoma and normal bile duct tissue were analyzed by using the GEPIA database tool. The expression levels of Nusap1 protein in 61 paired specimens of cholangiocarcinoma and adjacent tissue were determined by immunohistochemical technique, and the relations of Nusap1 protein expression with clinical parameters and the total survival time of the patients were analyzed. Results: The GEPIA database analysis showed that the Nusap1 mRNA was highly expressed in cholangiocarcinoma tissue compared to that in normal bile duct tissue (P<0.05). The results of immunohistochemical staining showed that the expression level of Nusap1 protein was significantly higher than that in the tumor adjacent tissue (47.5% vs. 14.8%, P<0.05). Moreover, the Nusap1 protein expression was significantly associated with the TNM stage (P=0.001), lymph node metastasis (P=0.011), and survival status (P=0.010). Kaplan-Meier analysis showed that the median survival time of patients with positive Nusap1 expression was 23 months and in those with negative Nusap1 expression was 45 months, and the postoperative survival time of the former was significantly shorter than that of the latter (P=0.000). Conclusion: Nusap1 expression is increased in cholangiocarcinoma tissue, and it may be closely related to the progression and poor prognosis of cholangiocarcinoma. Nusap1 may potentially become an indicator of prognosis and treatment target for cholangiocarcinoma.