COL12A1在胃癌中的表达及其生物学功能研究

目的:探讨COL12A1在胃癌中的表达水平和生物学功能。方法:利用Oncomine与GEPIA在线数据库分析COL12A1在胃癌中的表达及其与胃癌病理分期和预后的关系;用qPCR和免疫组化检测COL12A1基因与蛋白在58例胃癌及癌旁组织中的表达;用siRNA干扰技术敲低胃癌细胞SGC7901中COL12A1的表达后,通过CCK-8、平板克隆存活及Transwell小室实验分析SGC7901生物学行为的变化。结果:Oncomine与GEPIA在线数据库分析结果显示,COL12A1在胃癌中的表达水平明显升高(均P0.05);COL12A1表达与胃癌病理分期无明显关系(P0.05),但COL12A1高表达胃癌患者总体生存率明显降低(P0.05)。qPCR和免疫组化结果显示,胃癌组织中COL12A1基因与蛋白表达均明显高于其癌旁组织(均P0.05)。敲低COL12A1后,SGC7901的生长、增殖和侵袭能力明显降低(均P0.05)。结论:COL12A1在胃癌中高表达,其高表达可能促进胃癌细胞生长、增殖和侵袭,并与患者不良预后密切相关。

Expression of COL12A1 in gastric cancer and its biological functions

Objective: To investigate the expression of COL12A1 in gastric cancer and its biological function.   Methods: The COL12A1 expression in gastric cancer and its relations with pathological stage and prognosis of gastric cancer were analyzed by using Oncomine and GEPIA online databases. The gene and protein expressions of CLL12A1 in 58 specimens of gastric cancer and tumor adjacent tissue were determined by qPCR and immunohistochemical staining. In gastric cancer SGC7901 cells after CLL12A1 knockdown through siRNA interference technique, the changes in biological behaviors were observed through CCK-8 assay, plate clone formation assay, and Transwell chamber assay, respectively. Results: Oncomine- and GEPIA-based online analysis indicated that COL12A1 was significantly upregulated in gastric cancer (both P<0.05); COL12A1 expression was irrelevant to the pathological stage of gastric cancer (P>0.05), but the overall survival rate in gastric cancer patients with high COL12A1 expression was significantly decreased (P<0.05). the results of qPCR and immunohistochemical staining showed that both gene and protein expressions of COL12A1 were significantly higher than those in the tumor adjacent tissue (both P<0.05). In gastric cancer SGC7901 cells after CLL12A1 knockdown, the growth, proliferative and invasion abilities were all significantly inhibited (all P<0.05). Conclusion: COL12A1 is upregulated in gastric cancer, and high COL12A1 expression may probably promote growth, proliferative and invasion abilities of gastric cancer cells, and also related to poor prognosis of the patients.