乳腺癌组织中cIAP1、XIAP和caspase-3的表达及其临床意义

目的:探讨cIAP1、XIAP、caspase-3在乳腺癌组织中的表达与患者临床病理参数及预后的关系。方法:应用免疫组化法检测cIAP1、XIAP、caspase-3蛋白分子在99例乳腺癌、10例乳腺纤维腺瘤及6例癌旁正常乳腺组织中的表达,结合Kaplan-MeierPlotter数据库中的乳腺癌患者资料,分析3种蛋白与乳腺癌临床病理参数及其预后的关系。结果:免疫组化结果显示,乳腺癌组织中cIAP1、XIAP、caspase-3蛋白的表达水平均明显高于各自在正常乳腺组织中的表达(均P<0.05),但3种蛋白表达水平在乳腺癌组织与乳腺纤维腺瘤组织中均无统计学差异,且在接受过与未接受过新辅助化疗乳腺癌患者的癌组织中也无统计学差异(均P>0.05)。cIAP1的表达与患者的月经状态、年龄明显有关,而XIAP的表达与Ki-67的表达强度明显有关(均P<0.05)。Kaplan-MeierPlotter数据库分析结果显示,cIAP1表达量与乳腺癌患者的无复发生存期关系不明显(P>0.05),XIAP高表达乳腺癌患者的无复发生存期长于低表达乳腺癌患者,而caspase-3高表达乳腺癌患者的无复发生存期短于caspase-3低表达乳腺癌患者(均P<0.05)。结论:cIAP1、XIAP和caspase-3的表达可能与乳腺癌的发生、发展密切相关,XIAP和caspase-3可作为评估乳腺癌预后的潜在指标。

Expressions of cIAP1, XIAP and caspase-3 in breast cancer tissue and their clinical significance

Objective: To assess expressions of cIAP1, XIAP and caspase-3 in breast cancer tissue and their relations with clinicopathologic factors and prognosis.   Methods: The protein expressions of cIAP1, XIAP and caspase-3 in 99 specimens of breast cancer tissue, 10 specimens of breast fibroadenoma tissue and 6 specimens of tumor-adjacent normal mammary tissue were determined by immunohistochemical staining. The relations of the 3 proteins with clinicopathologic features and prognosis of breast cancer patients were analyzed, by combination with the data of breast cancer patients in Kaplan-Meier Plotter database. Results: The results of immunohistochemical staining showed that the expression levels of cIAP1, XIAP and caspase-3 protein in breast cancer tissue were all significantly higher than those in normal mammary tissue (all P<0.05), while no significant differences were noted in the expression levels of the 3 proteins between breast cancer tissue and breast adenofibroma tissue, and between breast cancer tissues from patients undergoing and not undergoing neoadjuvant chemotherapy (all P>0.05). The expression of cIAP1 was significantly associated with the menstrual status and age of patients, and the expression of XIAP was significantly related to the expression intensity of Ki-67 (all P<0.05). The results of Kaplan-Meier Plotter database analysis showed that the cIAP1 expression level exerted no significant influence on the relapse-free survival (RFS) time of breast cancer patients (P>0.05), while the RFS time in breast cancer patients with high XIAP expression level was longer than that in breast cancer patients with high XIAP expression level, and in patients with high caspase-3 expression level was shorter than that in those with low caspase-3 expression level (both P<0.05).  Conclusion: The expressions of cIAP1, XIAP and caspase-3 may be closely related to the occurrence and development of breast cancer, and XIAP and caspase-3 may potentially be prognostic indicators for breast cancer.