THE EFFECT OF CHYMOTRYPSIN ON THE DETERMINATION OF TOTAL ALPHA-GLUCOSIDASE ACTIVITY IN SEMINAL PLASMA AND THE CORRELATION BETWEEN ALPHA-GLUCOSIDASE LEVEL AND SEMEN PARAMETERS |
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Authors: | H.-R. Xu F. Chen Y.-F. Huang B. Yao N.-Q. Lu |
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Affiliation: | 1. Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, P. R. China;2. Life and Science College, Nanjing Normal University, Nanjing, P. R. China;3. Department of Microbiology and Immunology, Basic Medical College of Nanjing Medical University, Nanjing, P. R. China;4. Jiangsu Family Planning Research Institute, Nanjing, P. R. China |
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Abstract: | To evaluate the effect of chymotrypsin on the examination of alpha-glucosidase activity in seminal plasma, thirty-nine samples of fresh liquefied semen with or without chymotrypsin and forty-eight samples of fresh un-liquefied semen with chymotrypsin were determined for the total alpha-glucosidase activity in seminal plasma. The total alpha-glucosidase level of each sample was assayed by the method of glucose oxidase. The correlations between alpha-glucosidase level and semen parameters, including semen volume, pH, sperm concentration, grade a and b motility and total motility, were analyzed with SPSS 11.0 software. The results showed that chymotrypsin had no effect on seminal alpha-glucosidase activity determination. Chymotrypsin could improve the liquefaction for un-liquefied semen, and there was no significant difference of alpha-glucosidase activity between liquefied and un-liquefied semen samples. There were significantly positive correlations between seminal alpha-glucosidase activity (U/ml) and sperm concentration (r = 0.338, p = 0.015) and between total alpha-glucosidase activity (U/ejaculate) and semen volume (r = 0.677, p = 0.000). However, there was no significant correlation between alpha-glucosidase level (U/ml) and semen volume, pH, sperm motility or grade a and b motility (r = ?0.234 ~ 0.077, p = 0.099 ~ 0.993). The data indicated that chymotrypsin could be added into the un-liquefied semen samples for alpha-glucosidase activity determination, and there were different correlations between seminal alpha-glucosidase level and various semen parameters. |
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Keywords: | alpha-glucosidase chymotrypsin pH semen volume sperm concentration sperm motility |
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