miR-483与结直肠癌关系的研究

背景:胰岛素样生长因子2(IGF2)基因在多种恶性肿瘤中呈高表达,在肿瘤发生中起重要作用。miR-483位于IGF2基因第7号内含子中,与宿主基因共表达,但其具体功能尚未明确。目的:检测miR-483-3p、miR-483-5p在结直肠癌中的表达情况,探讨其作为结直肠癌分子标记物的可能性。方法:收集75对结直肠癌组织和相应癌旁非癌组织样本,以real-time PCR检测miR-483-3p、miR-483-5p表达并分析两者间以及两者与肿瘤临床病理特征的关系,以ROC曲线分析两者对结直肠癌的诊断性能。结果:结直肠癌组织中的miR-483-3p、miR-483-5p相对表达量均显著高于相应癌旁非癌组织(P0.000 1),且两者在癌组织中的表达量呈显著正相关(rs=0.554 5,P0.000 1)。miR-483-3p和miR-483-5p诊断结直肠癌的最佳截点分别为9.22和11.61,相应诊断敏感性、特异性分别为78.67%、62.67%和50.67%、85.33%;如两者联合检测(并联试验),诊断敏感性可提高至89.48%。两者表达与结直肠癌患者的性别、年龄以及肿瘤大小、部位、分化程度和TNM分期均无相关性。结论:miR-483-3p、miR-483-5p在结直肠癌中呈高表达,可能与肿瘤发生有关。两者有望成为结直肠癌诊断潜在的分子标记物。

Correlation of miR-483 with Colorectal Cancer

Background：Insulin-like growth factor 2 （IGF2） is overexpressed in many human cancers and might play an important role in the onset of cancer.miR-483 is located within the seventh intron of IGF2 gene and is coexpressed with the host gene.However,the function of miR-483 remains unclear.Aims：To study the expressions of miR-483-3p and miR-483-5p in colorectal cancer （CRC） and their potential value as biomarkers of CRC.Methods：Cancerous and adjacent non-cancerous tissues from 75 CRC patients were collected.Expressions of miR-483-3p and miR-483-5p were determined by real-time PCR,and the correlations between miR-483-3p,miR-483-5p and clinicopathological characteristics of CRC were analyzed.The diagnostic performance of the two miRNAs for CRC was assessed by ROC curve.Results：Expression levels of miR-483-3p and miR-483-5p were significantly higher in cancerous tissue than in adjacent non-cancerous tissue （P 〈 0.000 1）,and a significant positive correlation between the two miRNAs was seen in cancerous tissue （rs =0.554 5,P 〈 0.000 1）.The cutoff value of miR-483-3p for CRC was 9.22 with a sensitivity of 78.67% and a specificity of 62.67%,while those of miR-483-5p are 11.61,50.67% and 85.33%,respectively.When these two miRNAs were combined in parallel,a sensitivity of 89.48% could be achieved.No obvious correlations were found between miR-483-3p,miR-483-5p expressions and the gender,age,tumor size,location,differentiation and TNM staging of CRC.Conclusions：miR-483-3p and miR-483-5p are highly expressed in CRC and might be involved in cancer onset.They might be used as potential biomarkers for diagnosis of CRC.