氨溴索对急性肺损伤兔E-选择素和细胞间黏附分子-1的影响

目的观察氨溴索对油酸诱导的兔急性肺损伤E－选择素和ICAM－1的影响，探讨氨溴索抗急性肺损伤作用及其机制。方法健康日本大耳白兔24只，随机分为3组：生理盐水组（NS组），油酸组（OA组）。氨溴索治疗组（AMB组）。采用耳缘静脉注射复制兔油酸型急性肺损伤模型。检测各组动脉氧分压、肺组织湿干比（W／D），光镜观察肺组织病理改变；检测在静注氨澳索（AMB）或生理盐水6h后BALF和肺组织匀浆白细胞介素－1β（IL-1β）、肿瘤坏死因子－α（TNF-α）、E-选择素、细胞间黏附分子－1（ICAM－1）含量。结果AMB组肺W／D显著低于OA组（P〈0．01），但显著高于NS组（P〈0．05），肺组织病理损伤明显减轻；用药前AMB组和OA组PaO2显著低于NS组；用药后6小时，AMB组PaO2显著高于OA组（P〈0．01），但显著低于NS组（P〈0．01）。AMB组BALF和肺组织匀浆中IL-10、TNF-α、E-选择素及ICAM－1含量含量均显著低于OA组（P〈0．01），但均高于NS组（P〈0．01）。BALF和肺组织匀浆中E-选择素、ICAM－1含量与相应标本的IL－1β、TNF-α含量有高度的正相关关系（P〈0．001）。结论氮溴索有较好的抗急性肺损伤的作用。氨溴索能抑制促炎细胞因子等的释放及肺组织细胞黏附分子的合成，部分阻止多形核白细胞（PMN）与内皮细胞的粘附。进而阻止PMN的跨膜运动和其在肺内的聚集．从而发挥抗急性肺损伤的作用。

Effects of the ambroxol on E-selectin and intercellular adhesion molecule- 1 in acute lung injury of rabbits

OBJECTIVE To investigate the changes of E-selectin, intercellular adhesion molecule-1 of lung tissue during acute lung injury（ALI） induced by oleic acid. To determine the protection effects of ambroxol injection on acute lung injury and its mechanisms. METHODS The acute lung injury model of rabbit was induced by oleic acid.Twenty-four Japanese white rabbit were divided into three groups randomLy： normal saline group（NS group）, acute lung injury group（OA group）, acute lung injury plus ambroxol injection group （AMB group）. As the model of ALI was induced successfully, the rabbits in OA group and NS received normal saline, and in AMB group received ambroxol injection through auricular vein. The rabbits were sacrificed after 6 hours. The content of interleukin-1β （IL-1β）, tumor necrosis factor-α （TNF-α）, E-selectin, intercellular adhesion molecule-1 （ICAM- 1） in both bronchoalveolar lavage fluid （BALF） and lung tissue homogenate Were measured.The wet dry ratio （W/D） of lung tissue were tested.The pathological alternation in lung tissue were observed under microscope. RESULTS The W/D of lung tissue in AMB group was significant decreased compared with ALI group（P〈0.01）, but was significant higher than NS group（ P〈0.05）. Alveolitis were decreased compared with ALI group.The content of TNF-α , IL-1β, E-selectin and ICAM-1 both in BALF and in lung tissue homogenate in AMB group decreased significantly compared with ALI group（ P 〈0.01）, but were higher than NS group（ P〈 0.01） .The content of E-selectin, ICAM-1 correlated intensively with IL-1β, TNF-α in BALF or lung tissue homogenate respectively.CONCLUSIONS Ambroxol has protection effect on oleic acid-induced acute lung injury. Ambroxol could inhibit the release of proinflammatory cytokines （ie IL-1β and TNF-α） and inhibit the expression of cellular adhesion molecules（ie E-selectin and ICAM-1 ）, prevent the polymorphonuclear leukocytes from adhering endothelial cells and migratting to sites of