大肠癌VEGF-C和VEGF-D的表达及其相关性的研究

目的探讨血管内皮生长因子-C和-D(VEGF-C和-D)在大肠癌组织中的表达特点及其临床意义.方法应用免疫组化S-P法检测96例大肠癌及正常大肠组织中VEGF-C和-D的表达,并分析其与大肠癌临床病理参数及预后的关系.结果大肠癌组织VEGF-C和-D阳性率明显高于正常大肠组织(P＜0.01),而且两项指标间表达率呈显著正相关(r=0.466,P＜0.01).大肠癌VEGF-C或-D表达率在大肠癌分化程度、Dukes分期、淋巴结转移及患者预后间差异显著.(P＜0.01,P＜0.05).大肠癌原发灶与淋巴结转移灶中VEGF-C和-D表达相一致(P＜0.01,r=0.429和P＜0.01,r=0.511).结论大肠癌VEGF-C和-D高表达可能在肿瘤发生中起一定作用,并且可以作为判断大肠癌淋巴结转移和评估患者预后的重要参考指标.

Expression and clinical significance of vascular endothelial growth factors C and D in carcinomas of large intestine

Objective To explore the expression of endothelial growth factors C and D (VEGF-C and -D) in carcinomas of large intestine and its clinical significance. Methods The expression of VEGF-C and -D in 96 cases with carcinomas of large intestine were detected by SP immunohistochemical technique and its relationship with clinicopathological features and prognosis was analyzed. Results The expression of VEGF-C or -D was significantly higher in tumor tissue than that in normal mucosa (P<0.01). The expression of VEGF-C had significantly positive correlation with that of VEGF-D (r=0.466, P<0.01). VEGF-C and -D positive expression was significantly correlated with tumor differentiation, Dukes' stage, lymph node metastasis and prognosis in 96 cases with carcinomas of large intestine (P<0.05), but their relationship with age and gender of the patients, size, site and depth of tumor invasion and distant organ metastasis was not significant (P>0.05). The expression of VEGF-C and -D in metastatic lymph nodes was consistent with that in the primary tumor (r=0.429, P<0.01 and r=0.511, P<0.01). Conclusion VEGF-C and -D might be of great importance in colorectal carcinogenesis because of their overexpression in carcinoma of large intestine, and they may serve as useful markers to predict lymph node metastasis and to evaluate prognosis of patients with CRC.