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| Crohn＇s disease in Japanese is associated with a SNP-haplotype of N-acetyltransferase 2 gene | |
| 引用本文： | Machida H,Tsukamoto K,Wen CY,Shikuwa S,Isomoto H,Mizuta Y,Takeshima F,Murase K,Matsumoto N,Murata I,Kohno S,Wen CY. Crohn＇s disease in Japanese is associated with a SNP-haplotype of N-acetyltransferase 2 gene[J]. World journal of gastroenterology : WJG, 2005, 11(31): 4833-4837. DOI: 10.3748/wjg.v11.i31.4833 |
| 作者姓名： | Machida H Tsukamoto K Wen CY Shikuwa S Isomoto H Mizuta Y Takeshima F Murase K Matsumoto N Murata I Kohno S Wen CY |
| 作者单位： | Second Department of Internal Medicine,Department of Pharmaco-therapeutics,Department of Gastroenterology,Second Department of Internal Medicine,Second Department of Internal Medicine,Second Department of Internal Medicine,Second Department of Internal Medicine,Department of Human Genetics,Department of Pharmaco-therapeutics,Naomichi Matsumoto Nagasaki University School of Medicine,1-7-1 Sakamoto,Nagasaki 852-8501,Japan,Nagasaki University Graduate School of Biomedical Sciences,1-14 Bunkyo-machi,Nagasaki 852-8521,Japan,National Hospital Organization Nagasaki Medical Center,2-1001-1 Kubara,Omura 856-8562,Japan,Nagasaki University School of Medicine,1-7-1 Sakamoto,Nagasaki 852-8501,Japan,Nagasaki University School of Medicine,1-7-1 Sakamoto,Nagasaki 852-8501,Japan,Nagasaki University School of Medicine,1-7-1 Sakamoto,Nagasaki 852-8501,Japan,Nagasaki University School of Medicine,1-7-1 Sakamoto,Nagasaki 852-8501,Japan,Nagasaki University Graduate School of Biomedical Sciences,1-12-4 Sakamoto,Nagasaki 852-8523,Japan,Nagasaki University Graduate School of Biomedical Sciences,1-14 Bunkyo-machi,Nagasaki 852-8521,Japan,CREST,JST,Kawaguchi,Japan |
| 摘 要： | AIM: To investigate the frequency and distribution of N-acetyltransferase 2 (NAT2) and uridine 5'-diphosphate (UDP)-glucuronosyltransferase 1A7 (UGT1A7) genes in patients with ulcerative colitis (UC) and Crohn's disease (CO). METHODS: Frequencies and distributions of NAT2 and UGT1A7SNPs as well as their haplotypes were investigated in 95 patients with UC, 60 patients with CD, and 200 gender-matched, unrelated, healthy, control volunteers by PCR-restriction fragment length polymorphism (RFLP), PCR-denaturing high-performance liquid chromatography (DHPLC), and direct DNA sequencing. RESULTS: Multiple logistic regression analysis revealed that the frequency of haplotype, NAT2*7B, significantly increased in CD patients, compared to that in controls (P=0.0130, OR = 2.802,95%CI = 1.243-6.316). However, there was no association between NAT2 haplotypes and UC, or between any UGT1A7 haplotypes and inflammatory bowel disease (IBD). CONCLUSION: It is likely that the NAT2 gene is one of the determinants for CD in Japanese. Alternatively, a new CD determinant may exist in the 8p22 region, where NAT2 is located. |
| 关 键 词： | 克隆氏病 肠疾病 日本 单模标本 N-乙酰基转移酶2 基因表达 |
| 收稿时间： | 2004-12-20 |
Crohn's disease in Japanese is associated with a SNP-haplotype of N-acetyltransferase 2 gene |
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| Machida Haruhisa,Tsukamoto Kazuhiro,Wen Chun-Yang,Shikuwa Saburou,Isomoto Hajime,Mizuta Yohei,Takeshima Fuminao,Murase Kunihiko,Matsumoto Naomichi,Murata Ikuo,Kohno Shigeru,Wen Chen-Yang. Crohn's disease in Japanese is associated with a SNP-haplotype of N-acetyltransferase 2 gene[J]. World journal of gastroenterology : WJG, 2005, 11(31): 4833-4837. DOI: 10.3748/wjg.v11.i31.4833 | |
| Authors: | Machida Haruhisa Tsukamoto Kazuhiro Wen Chun-Yang Shikuwa Saburou Isomoto Hajime Mizuta Yohei Takeshima Fuminao Murase Kunihiko Matsumoto Naomichi Murata Ikuo Kohno Shigeru Wen Chen-Yang |
| Affiliation: | 1. Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan 2. Department of Pharmaco therapeutics, Nagasaki University Graduate School of Biomedical Sciences, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan;CREST, JST,Kawaguchi, Japan 3. Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan 4. Department of Gastroenterology, National Hospital Organization Nagasaki Medical Center, 2-1001-1 Kubara,Omura 856-8562, Japan 5. Department of Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto,Nagasaki 852-8523, Japan 6. Departnent of Digestive Disease, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China |
| Abstract: | AIM: To investigate the frequency and distribution of N-acetyltransferase 2 (NAT2) and uridine 5'-diphosphate (UDP)-glucuronosyltransferase 1A7 (UGT1A7) genes in patients with ulcerative colitis (UC) and Crohn's disease (CO). METHODS: Frequencies and distributions of NAT2 and UGT1A7SNPs as well as their haplotypes were investigated in 95 patients with UC, 60 patients with CD, and 200 gender-matched, unrelated, healthy, control volunteers by PCR-restriction fragment length polymorphism (RFLP), PCR-denaturing high-performance liquid chromatography (DHPLC), and direct DNA sequencing. RESULTS: Multiple logistic regression analysis revealed that the frequency of haplotype, NAT2*7B, significantly increased in CD patients, compared to that in controls (P=0.0130, OR = 2.802,95%CI = 1.243-6.316). However, there was no association between NAT2 haplotypes and UC, or between any UGT1A7 haplotypes and inflammatory bowel disease (IBD). CONCLUSION: It is likely that the NAT2 gene is one of the determinants for CD in Japanese. Alternatively, a new CD determinant may exist in the 8p22 region, where NAT2 is located. |
| Keywords: | Crohn's disease N-acetyltransferase 2 gene Polymorphism Disease-susceptible gene Association study Japanese population |
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