结核分枝杆菌Rv3134c蛋白抗原表位的预测

目的 预测结核分枝杆菌Rv3134c的抗原表位,了解其免疫学特性。方法 利用DNAStar软件包中Protean软件对Rv3134c氨基酸序列进行分析,采用包括二级结构、亲水性、抗原性、表面可能性、柔韧性等多参数预测其二级结构及T细胞和B细胞抗原表位,最后BLAST分析其与人类抗原表位的同源性。结果 Rv3134c蛋白具有丰富的二级结构和多处抗原指数较高的区段,含有较多潜在的B细胞抗原表位,可能位于1-7、30-37、65-81、89-100、111-120、138-148、184-195、209-216、233-238位氨基酸残基或其附近,这些区域基本上含有β转角结构,亲水性、表面可能性和柔韧性指数都较高。该蛋白潜在的T细胞抗原表位较少,可能位于62-76、89-97、185-195、203-213、219-231、248-255位氨基酸残基或其附近。BLAST分析结果显示,其与人类抗原表位的同源性很低。结论 结核分枝杆菌Rv3134c是一个即含有较多B细胞抗原表位又含有较多T细胞抗原表位的蛋白抗原,实验结果为该蛋白抗原表位的进一步研究与合成肽疫苗奠定了基础。

Prediction of epitopes of Mycobacterium tuberculosis Rv3134c protein

In this study, we predicted the epitopes of Mycobacterium tuberculosis Rv3134c protein and investigated its immunological characteristics. The amino acid sequence of Rv3134c protein was input and predicted the epitopes of B cell and T cell using multi-parameters including the secondary structure, hydrophilicity, antigenicity, accessibility, flexibility, charge distribution by Protean software of DNAStar software package. Results showed that the Rv3134c protein had rich secondary structure and multiple sections with higher antigenicity. There were a few potential B cell epitopes at 1-7, 30-37, 65-81, 89-100, 111-120, 138-148, 184-195, 209-216, and 233-238 amino acid residues or nearby, and theses epitopes had better antigenicity, contained beta angle and irregular coil structure, presented in the surface probability and had larger flexibility. There also were more potential T cell epitopes of the protein containing at 62-76, 89-97, 185-195, 203-213, 219-231, 248-255, amino acid residues or nearby. There was low homology between Rv3134c protein and human protein by BLAST analysis. In conclusion, Mycobacterium tuberculosis Rv3134c protein had more B-cell epitopes and T-cell epitopes, which will lay the foundation for its further study and the development of vaccine.