| 重组人生长激素对脑缺血/再灌注损伤细胞凋亡及Nestin表达的影响 |
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| 引用本文： | 刘海涛,白宏英,曾志磊,郭耀强,方李鸿.重组人生长激素对脑缺血/再灌注损伤细胞凋亡及Nestin表达的影响[J].中国实用神经疾病杂志,2010,13(1):39-41. |
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| 作者姓名： | 刘海涛 白宏英 曾志磊 郭耀强 方李鸿 |
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| 作者单位： | 郑州大学第二附属医院神经内科,郑州,450014 |
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| 摘 要： | 目的观察重组人生长激素(recombinant human growth hormone,rhGH)对脑缺血/再灌注(ischemia-reperfu-sion,I/R)损伤后神经元细胞凋亡及Nestin表达的影响。方法54只SD雄性大鼠随机分成3组:假手术组、对照组(脑缺血/再灌注+生理盐水组)、治疗组(脑缺血/再灌注+重组人生长激素),每组18只。应用线栓法制作大鼠脑缺血/再灌注损伤模型,大脑中动脉缺血2h,分别在再灌注7d、14d、21d后处死,采用TUNEL法,免疫组织化学法检测顶叶皮质内7d、14d、21d的神经元凋亡和巢蛋白(Nestin)的表达。结果应用重组人生长激素干预后顶叶皮质在7d、14d、21d神经元凋亡数较大鼠对照组明显减少,Nestin的表达显著增加。结论rhGH的神经保护作用可能与抑制缺血神经元细胞凋亡和上调Nestin的表达有关。
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| 关 键 词： | 缺血/再灌注 TUNEL 重组人生长激素 Nestin |
Effects of recombinant human growth hormone on apoptosis and expression of Nestin in rats with cerebral ischemia-reperfusion injury |
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| Liu Haitao,Bai Hongying,Zeng Zhilei,et al.Effects of recombinant human growth hormone on apoptosis and expression of Nestin in rats with cerebral ischemia-reperfusion injury[J].Chinese Journal of Practical Neruous Diseases,2010,13(1):39-41. |
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| Authors: | Liu Haitao Bai Hongying Zeng Zhilei |
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| Institution: | Liu Haitao,Bai Hongying,Zeng Zhilei,et al.Department of Neurology,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China |
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| Abstract: | Objective To investigate the effect of the recombinant human growth hormone(rhGH) on apoptosis and the expression of Nestin in male SD rats with cerebral ischemia-reperfusion injury.Methods The middle cerebral artery ischemia-reperfusion injury model was established by middle cerebral artery occlusion(MCAO) with nylon suture.Fifty-four rats were randomly divided into 3 groups: the sham group(n=18),the control group(n=18) and the treatment group(n=18).We used TUNEL and immunohistochemical method to detect TU... |
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| Keywords: | Ischemia-reperfusion TUNEL rhGH Nestin |
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