| 膀胱移行细胞癌中P16基因的甲基化失活 |
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| 引用本文： | 武玉东,魏金星,李中凌,李凤云. 膀胱移行细胞癌中P16基因的甲基化失活[J]. 中国伤残医学, 2001, 9(1): 24-27 |
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| 作者姓名： | 武玉东 魏金星 李中凌 李凤云 |
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| 作者单位： | 1. 河南医科大学第一附属医院泌尿外科
2. 河南油田职工医院 |
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| 摘 要： | 目的研究膀胱移行细胞癌中p16基因甲基化失活及其在肿瘤发生中的作用.方法应用DNA甲基化分析技术检测52例膀胱移行细胞癌中P16基因5'CPG岛的甲基化状态，应用RT-PCR检p16基因mRNA的表达.结果52例膀胱移行细胞癌中19例出现p16基因5'CPG岛的甲基化，甲基化率为36.54％，而发生甲基化的肿瘤均未检测p16mRNA表达.P16基因5'CPG岛甲基化多见于高期、高级肿瘤(P＜0.05).结论P16基因5'CPG岛的甲基化引起的基因失活是膀胱肿瘤发展过程中的晚期所见.
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| 关 键 词： | 膀胱肿瘤 癌 基因 |
| 修稿时间： | 2000-08-12 |
Methylation Inactivationof P16 Gene in Human Bladder Cancer |
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| Affiliation: | Department of Urology,First Affiliated Hospital of Hena Medical University |
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| Abstract: | objective: To investigate the role of p16 gene aberrant methylation in the malignant progression of human bladder cancer. Methods: p16 gene 5'CPG island methylation and mRNA expression of 52 cases bladder cancer were detected by DNA methylation analysis and RT-PCR respectively. Results: p16 gene 5'CPG island methylation was found in 19 of 52 (36.5%) cases of bladder cancer. There was no p16 gene mRNA expression in this tumor. p16 gene 5'CPG island methylation was significantly associated with tumor of higher grade and stage (P<0.05). Conclusion: p16 gene inactivation by 5'CPG island methylation might be a late event in the malignant progression of human bladder cancer. |
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| Keywords: | Bladder neoplasm Carcinoma Gene |
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