|
|||||
|
|
| 胆脂瘤患者外耳道上皮细胞增殖和凋亡的研究 | |
| 引用本文： | 井玲,祝威,黄冬雁,杜宝东.胆脂瘤患者外耳道上皮细胞增殖和凋亡的研究[J].吉林大学学报(医学版),2003,29(6):742-744. |
| 作者姓名： | 井玲 祝威 黄冬雁 杜宝东 |
| 作者单位： | 1. 吉林大学再生医学科学研究所病理学研究室,吉林,长春,130021 2. 吉林大学第一医院耳鼻喉科 3. 解放军第301医院耳鼻喉科 |
| 基金项目： | 卫生部地方病司跨世纪优秀人才培养计划;1999-2001; |
| 摘 要： | 目的 :检测胆脂瘤上皮及胆脂瘤患者外耳道上皮增殖细胞核抗原 ( PCNA)、Ki- 67抗原和凋亡细胞。方法 :取后天继发性中耳胆脂瘤患者和正常人外耳道皮肤 (各 5例 )及胆脂瘤上皮 ( 1 3例 )。应用免疫组化和原位切口末端标记 ( TUNUL)法检查 PCNA、Ki- 67抗原和凋亡细胞的分布和数量。结果 :胆脂瘤患者外耳道皮肤 PCNA、Ki- 67抗原的分布和数量与胆脂瘤上皮相似 ,而较正常外耳道上皮分布范围广 ,阳性细胞多且深染 ;凋亡细胞数量与正常外耳道上皮相比有明显增加趋势。结论 :外耳道上皮异常增殖和细胞凋亡可能与中耳胆脂瘤的发生有关 |
| 关 键 词： | 胆脂瘤 中耳 增殖细胞核抗原 Ki-67抗原 细胞凋亡 免疫组织化学 原位缺口末端标记 |
| 文章编号： | 1671-587X(2003)06-0742-03 |
| 修稿时间： | 2002年12月9日 |
Study on the Relationship between the Proliferation and Apoptosis of Auditory Meatal Skin in Cholesteatoma |
|
| JING Ling ,ZHU Wei ,HUANG Dong yan ,DU Bao dong.Study on the Relationship between the Proliferation and Apoptosis of Auditory Meatal Skin in Cholesteatoma[J].Journal of Jilin University: Med Ed,2003,29(6):742-744. | |
| Authors: | JING Ling ZHU Wei HUANG Dong yan DU Bao dong |
| Institution: | JING Ling 1,ZHU Wei 2,HUANG Dong yan 3,DU Bao dong 2 |
| Abstract: | Objective:To study on the relationship between the proliferation and apoptosis of auditory meatal skin in patients with cholesteatoma. Methods:Five samples of auditory meatal skin in cholesteatoma, 5 samples of normal auditory meatal skin and 13 samples of cholesteatoma epithelium were selected. Immunohistochemistry analysis and TUNEL method were used in the experiment. Results:A number of positive cells were observed in the basal and suprabasal layers in auditory meatal skin samples of cholesteatoma and in epithelium of cholesteatoma than those in normal auditory meatal skin. An increasing tendency of apoptotic cells was seen in the outmost layer of auditory meatal skin samples of cholesteatoma. Conclusion:The results surggested that the disturbance of proliferation and cell apoptosis in auditory meatal skin in patients suffering from cholesteatoma may play an important role in the pathogenesis of middle ear cholesteatoma. |
| Keywords: | Cholesteatoma middle ear Proliferating cell nuclear antigen Ki 67 antigen Apoptosis Immunohistochemistry In situ nick end labeling |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |