瘦素对人卵巢黄素化颗粒细胞雌、孕激素分泌的体外实验研究

目的探讨瘦素在体外对人卵巢颗粒细胞雌激素和孕激素生成的影响。方法将来自体外受精-胚胎移植（IVF-ET）的卵巢黄素化颗粒细胞纯化后，在不同浓度瘦素(0、10、30、100、300ng/ml)和人绝经期促性腺激素（hMG，0、0.1、0.2、0.5、1、2、5、10IU/ml）单独或联合作用下进行体外培养，收集培养液，采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。结果不同浓度的瘦素对雌二醇、孕酮的生成无影响；hMG为5IU/ml时，雌二醇水平平均为2.36×10-11mol/L，加入不同浓度瘦素（10、30、100、300ng/ml），雌二醇的生成均受到明显的抑制，随瘦素浓度增加，雌二醇水平逐渐降低（P<0.05），孕酮水平无明显变化；hMG浓度小于0.5IU/ml时，瘦素对雌二醇生成无影响；hMG浓度大于0.5IU/ml、瘦素为100ng/ml时，雌二醇水平低于不加瘦素的对照（P<0.05）。结论瘦素参与卵巢颗粒细胞激素生成的调节，在卵泡发育和黄体形成中有一定的作用。

Effects of leptin on estradiol and progesterone production by human luteinized granulosa cells in vitro

OBJECTIVE: To investigate the effects of leptin on steroidogenesis of human luteinized granulosa cell in vitro. METHODS: Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml), human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 micrograms/ml. For 2 days the media were collected for estradiol and progesterone measurements. RESULTS: Addition of leptin alone did not alter estradiol and progesterone production (P > 0.05) by human luteinized granulosa cells. Leptin of 10-30 ng/ml concentrations caused a dose-dependent inhibition of estradiol production (P < 0.05) while greater than 0.5 IU/ml of hMG were added. There was no effect of leptin on hMG-stimulated progesterone production (P > 0.05). CONCLUSION: Leptin can directly inhibit hMG-stimulated estradiol production by human luteinized granulosa cells in vitro, but has no effect on progesterone production. Leptin may play an important role in follicle development and luteinization.