同种异体移植近期神经再生的实验研究

目的观察犬去细胞异体神经移植的近期神经再生.方法15犬分成去细胞神经移植组6犬、自体神经移植组6犬、新鲜异体神经移植组3犬.坐骨神经5.0cm长缺损,以3种神经移植物桥接修复.术后6个月行神经再生的组织学观察.结果移植段内大量新生神经纤维,新生血管及许旺细胞;远端胫神经内大量的有髓神经纤维;靶肌肉运动终板的数量、形态及分布良好.去细胞神经移植组和自体神经移植组非常相似.结论化学去细胞神经作为同种异体神经移植物,在修复犬的粗大和长段神经缺损时不会被宿主排斥和吸收,其神经再生与自体神经移植无明显差别.

Nerve regenerationafter allografting

AIM: To observe the nerve regeneration after acellular allografting in canines.METHODS: Fifteen canines were divided into acellular nerve allografting group(ANG, 6 canines), autografting group(AG, 6 canines) and fresh nerveallografting group(FNG, 3 canines). The sciatic nerves on the right side of all animals were exposed and a 5.0 cm-long segment in the mid-thigh level were removed and replaced by the three types of grafts. The nerve regeneration was observed by morphological study at 6 months after operations.RESULTS: There were many newborn nerve fibers, newborn vessels and Schwann cells in the transplanted segment. There were many myelinated nerve fibers in tibial nerve at the distal end. The quantity, shape and distribution of motor endplate in the target muscle were good. The results of ANG and AG groups were similar.CONCLUSION: Chemical acellular nerve, as an allograft, would not be rejected and absorbed by the host in the restoration of thick and long-segmental nerve defect in canines, of which the nerve regeneration had no significant difference compared with that of nerve autografting.