| 产肠毒素大肠杆菌耐热性肠毒素(ST)单克隆抗体研制及应用 |
| |
| 引用本文： | 黄晓哲,沈健民,谢梅雯,虞丽琴,沈鼎鸿,陆德源,司马惠兰. 产肠毒素大肠杆菌耐热性肠毒素(ST)单克隆抗体研制及应用[J]. 中国公共卫生学报, 1991, 0(5) |
| |
| 作者姓名： | 黄晓哲 沈健民 谢梅雯 虞丽琴 沈鼎鸿 陆德源 司马惠兰 |
| |
| 作者单位： | 上海市卫生防疫站 200031 |
| |
| 摘 要： | 本研究用小分子量大肠杆菌耐热性肠毒素(ST)经戊二醛聚合成功地免疫了Balb/c小鼠,小鼠脾细胞与SP2/O小鼠骨髓瘤细胞融合,获得1株产生特异性ST单克隆抗体(STMcAb)的杂交瘤细胞株并用特的STMcAb建立了检测ETEc ST的竞争性ELISA。该方法检测ST的敏感性较乳鼠灌胃法(SMA)高30倍。用两种方法对330株腹泻监测点的大肠杆菌做平行试验,发现12个相同的菌株两种试验ST均阳性,其余菌株两种试验均阴性。
|
| 关 键 词： | 产肠毒素大肠杆菌 耐热性肠毒素 单克隆抗体 |
Preparation and Application of E. coli Heat-stable Enterotoxin McAb |
| |
| Huang Xiaozhe,et al.. Preparation and Application of E. coli Heat-stable Enterotoxin McAb[J]. , 1991, 0(5) |
| |
| Authors: | Huang Xiaozhe et al. |
| |
| Affiliation: | Huang Xiaozhe,et al.,Shanghai Hygiene and Anti-epidemic Center 200031 |
| |
| Abstract: | In this test, the Balb/c mice was immuned su- ccessfully by using small molecule ST polymerised with glutaraldehyde. The spleen cells of the mice are fused with SP2/0 myeloma cells and one hybr- idoma strain which produces STMcAb is obtained. We have established 2 competetive ELISA by using the STMcAb for detecting ETEC ST. This method is 30 times more sensitive than SMA. A total of 330 E. coli strains are detected by competetive EL- ISA and SMA. There are 12 strains to be found ST positive and the rest strains are all negative by the two methodes. |
| |
| Keywords: | Enterotoxigenic Escherichia coli Heat-stable enterotoxin Monocolonal antibody |
| 本文献已被 CNKI 等数据库收录！ |
|
