| 免疫斑点法测定A族链球菌表面IgG Fc受体 |
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| 引用本文： | 沈叙庄,袁林.免疫斑点法测定A族链球菌表面IgG Fc受体[J].中华微生物学和免疫学杂志,1997,17(2):149-151. |
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| 作者姓名： | 沈叙庄 袁林 |
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| 作者单位： | 北京儿童医院 |
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| 基金项目： | 国家自然科学基金,北京市自然科学基金 |
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| 摘 要： | 本研究采用辣根过氧化物酶标记免疫球蛋白Ｇ，以免疫斑点法直接测定１４１各种４族链球菌感染性疾病临床分离株表面ＩｇＧ，Ｆｃ段受体含量。结果表明：１．这种方法敏感生高，重复性好，操作简便，结果判断直观且可定量。２．９８％的菌株可测到不同疾病，不同Ｍ型的混浊因子，阳性和阴性菌株之间其受体含量的差异无显著性意义，提示；酶标ＩｇＧ直接免疫斑点法是测定ＧＡＳ表面ＩｇＧ Ｆｃ受体的较好的实验方法；感染菌株Ｆｃ受体
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| 关 键 词： | 酿脓 链球菌 免疫球蛋白类 Fc 免疫斑点法 |
Immunoglobulin G Fc receptor of Group A Streptococci detected with immunoblot assay |
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| Abstract: | The contents of IgG Fc receptor of 141 Group A Streptococcal isolates were detected with immunoblot,an assay based on the binding of enzyme labelled immunoglobulins G.The results showed:IgG Fc receptor could be found among 98% of all isolates.The contents of IgG Fc receptor had no difference among different M/OF strains or strians from different kinds of diseases.This method offered several advatages over previous ones:High sensitivity,good repetition,allowing tests to be performed quickly and easily,result being determined visually.It indicated that this method was better than other ones in detecting IgG Fc receptor of Group A Streptococci.It is necessary to study more on the relationship of the contents of IgG Fc receptor among different kinds of diseases and M/OF types. |
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| Keywords: | Streptococcus pyogenes Immunoglobulins Fc Receptors Fc Immunoassay |
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