| 二陈汤合桃红四物汤含药血清对ox-LDL诱导内皮细胞损伤的保护作用及机制 |
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| 引用本文： | 王俊岩,曲宁宁,贾连群,陈文娜,张林,宋囡,杨关林,冼绍祥.二陈汤合桃红四物汤含药血清对ox-LDL诱导内皮细胞损伤的保护作用及机制[J].中国动脉硬化杂志,2018,26(10):987-992. |
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| 作者姓名： | 王俊岩 曲宁宁 贾连群 陈文娜 张林 宋囡 杨关林 冼绍祥 |
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| 作者单位： | 广州中医药大学第一临床医学院;辽宁中医药大学中医脏象理论及应用教育部重点实验室;广州中医药大学第一附属医院 |
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| 基金项目： | 国家自然科学基金项目(81774022、81300229)；国家重点基础研究发展计划项目(973计划)(2013CB531704) |
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| 摘 要： | 目的观察二陈汤合桃红四物汤含药血清对氧化型低密度脂蛋白(ox-LDL)诱导内皮细胞损伤的保护作用并探讨其可能的机制。方法培养EA.hy926细胞,随机分为对照组、ox-LDL组、干预组、抑制剂组。流式细胞仪检测各组细胞凋亡水平,比色法检测细胞内丙二醛(MDA)、一氧化氮(NO)水平及超氧化物歧化酶(SOD)活性,ELISA检测细胞上清液中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平,Western blot检测磷脂酰肌醇3-激酶(PI3K)、磷酸化AKT(p-AKT)、内皮型一氧化氮合酶(eNOS)蛋白表达水平。结果与对照组比较,ox-LDL组细胞上清液中TNF-α和IL-6水平显著升高(P0.01),细胞内MDA含量显著升高(P0.01),SOD活性明显降低(P0.05),上清液中NO水平显著降低(P0.01),活性氧(ROS)含量和细胞凋亡率明显升高,PI3K、eNOS蛋白表达水平和AKT蛋白磷酸化水平显著降低(P0.01)。与ox-LDL组比较,干预组细胞上清液中TNF-α和IL-6水平显著降低(P0.01),细胞内MDA含量显著降低(P0.01),SOD活性明显升高(P0.05),上清液中NO水平显著升高(P0.01),ROS含量和细胞凋亡率明显降低,PI3K、eNOS蛋白表达水平和AKT蛋白磷酸化水平显著升高(P 0.01)。与干预组比较,抑制剂组细胞上清液中TNF-α和IL-6水平显著升高(P0.01),细胞内MDA含量显著升高(P0.01)、SOD活性明显降低(P0.05),上清液中NO水平明显降低(P0.05),细胞内ROS含量升高,PI3K、eNOS蛋白表达水平和AKT蛋白磷酸化水平显著降低(P0.01)。结论二陈汤合桃红四物汤含药血清能有效保护ox-LDL诱导的内皮细胞损伤,其机制可能与激活PI3K/AKT/eNOS信号通路有关。
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| 关 键 词： | 二陈汤 桃红四物汤 氧化型低密度脂蛋白 内皮细胞 动脉粥样硬化 |
| 收稿时间： | 2018/4/19 0:00:00 |
| 修稿时间： | 2018/5/17 0:00:00 |
Effect and mechanism of serum containing of Erchen decoction and Taohong Siwu decoction on ox-LDL induced endothelial cell damage |
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| WANG Junyan,QU Ningning,JIA Lianqun,CHEN Wenn,ZHANG Lin,SONG Nan,YANG Guanlin and XIAN Shaoxiang.Effect and mechanism of serum containing of Erchen decoction and Taohong Siwu decoction on ox-LDL induced endothelial cell damage[J].Chinese Journal of Arteriosclerosis,2018,26(10):987-992. |
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| Authors: | WANG Junyan QU Ningning JIA Lianqun CHEN Wenn ZHANG Lin SONG Nan YANG Guanlin and XIAN Shaoxiang |
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| Institution: | The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China,Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Ministry of Education of China, Shenyang, Liaoning 110847, China,Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Ministry of Education of China, Shenyang, Liaoning 110847, China,Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Ministry of Education of China, Shenyang, Liaoning 110847, China,Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Ministry of Education of China, Shenyang, Liaoning 110847, China,Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Ministry of Education of China, Shenyang, Liaoning 110847, China,Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Ministry of Education of China, Shenyang, Liaoning 110847, China and The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, China |
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| Abstract: | |
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| Keywords: | Erchen decoction Taohong Siwu decoctionm oxidized low density lipoprotein endothelial cells atherosclerosis |
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