不同剂量蛇毒抗高凝状态酶对小鼠肝癌H22生长作用的实验研究

目的 :探讨不同剂量蛇毒抗高凝状态酶 (AHCSE)对小鼠肝癌H2 2生长作用的影响。方法 :小鼠皮下接种小鼠肝癌(H2 2 )后 ,待肿瘤长成 1 0mm2 分别经腹腔注射不同剂量AHCSE,每天 1次 ,连续 1 0d ,观察瘤重抑制率、瘤体积、抑瘤率、脾指数和白细胞及其组成成分 ,统计采用LDS t,检验水准α =0 .0 5。采用白细胞及其组分与脾指数的相关和回归。结果 :中、高剂量组AHCSE具有明显的抗肿瘤作用 ,而低剂量组抗肿瘤作用效果不佳 ,但高剂量组的小鼠体重降低明显。实验组的脾系数明显高于对照组 ,并且随着AHCSE的剂量增大脾系数也增大。白细胞随着AHCSE剂量的增大而增大 ,白细胞及其组分与脾指数存在相关性和回归直线。结论 :在一定的范围内 ,随着AHCSE的剂量增大 ,抗肿瘤效果增强 ,但副作用也增大 ,其机制可能与凋亡和激活免疫系统有关

Experimental study on inhibitory effect of antihypercoagulability state enzyme of snake venom in different dosages in mice with Hepatocarcinoma

AIM:To research the inhibitory effect of antihypercoagulability state enzyme (AHCSE) of snake venom in different dosages in mice with Hepatocarcinoma. METHODS: Hepatic cancer H22 model in mice was performed through subcutaneous inoculation of Right-Sidedness Axilla of BALB/C mice. Different dosages of AHCSE (1, 2, and 4 mg*kg-1) were injected via abdominal cavity everyday for 10 days. When the tumor grew to 10 mm3, the weight, volume, percent inhibition of tumor, spleen index and the number and the component of white blood cells were observed. RESULTS: The middle and high dosages of AHCSE had obvious inhibition in hepatic cancer. It showed a significant dose-dependent relation in the effects of AHCSE on neoplastic weight, neoplastic volume and splenic index. There was a linear regression between the number of leucocytes and the splenic index. CONCLUSION: AHCSE of 2-4 mg*kg-1 has significant inhibitory effect on tumor, and shows dose-related effect in mice with Hepatocarcinoma. Its mechanism is presumably associated with activating immune system.