5/6 肾切除大鼠模型尿纤溶酶原激活物活性的动态观察

了解５／６肾切除大鼠尿纤溶酶原激活性排泄量的动态变化。方法：５／６肾切除术后不同时间点分别检测尿ＰＡ活性以及血，尿生化尿生指标，并进行肾脏光镜，电镜检查。结果术后２周血肌酐显著升高但肾脏病理改变轻微，多数大鼠水ＰＡ排泄量仍能维持正常水平。

Dynamic observation of urinary plasminogen activator excretion in 5/6 nephrectomized rats

Objective:to observe the urinary plasminogen activator (PA) excretion in 5/6 nephrectomized rats. Methods:Light and electronic microscopic examination of the kidney were carried out at different time points after 5/6 nephrectomy, and urinary PA excretion rate as well as blood and urine biochemical parameters were dynamically observed.Results: Two weeks after 5/6 nephrectomy, acute renal failure induced by renal mass loss occurred, but renal pathologic changes was minimal. In this stage, the urinary PA activity of some rats decreased, but that of the others maintained normal level despite the renal mass loss. Four weeks after 5/6 nephrectomy, glomerular hypertrophy and mesangial proliferation became the characterized pathological changes,and the serum creatinine was nearly normal. In this stage, urinary PA activity decreased in some, and normalized or even increased in the others. Six to eight weeks after 5/6 nephrectomy, chronic renal insufficiency and heavey proteinuria occurred, renal pathology was characterized by focal and segmental glomerulosclerosis, as well as progressive tubulo interstitial changes. In this stage, urinary PA excretion increased gradually, and total urinary PA output increased significantly at 8 weeks after 5/6 nephrectomy comparing with the sham operated group, P <0 05.Conclusion:5/6 nephrectomy might trigger the compensatory increase of PA excretion in urine.