Taxifolin mitigates oxidative DNA damage in vitro and protects zebrafish (Danio rerio) embryos against cadmium toxicity |
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| Affiliation: | 1. Department of Biotechnology, School of Biosciences, Periyar University, Salem, 636011, Tamilnadu, India;2. Harborview Research & Training Building, University of Washington School of Medicine;1. Department of Veterinary Physiology, College of Veterinary Medicine, Chonbuk National University, 664-14, 1GA Deokjin-Dong, Deokjin-Gu, Jeonju City, Jeollabuk-Do 561-756, Republic of Korea;2. Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, 600034, India;1. Department of Biotechnology, Vinoba Bhave University, Hazaribagh, Jharkhand, India;2. Advance Science and Technology Research Centre, Vinoba Bhave University, Hazaribagh, India;3. Division of Pediatric Hematology and Oncology, University Children’s Hospital, University of Freiburg, 79106, Germany;4. School of Biotechnology, KIIT University, Bhubaneswar, Odisha, India;1. Academy for Engineering & Technology, Fudan University, Shanghai 200433, PR China;2. CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, PR China;3. School of Biology & Basic Medical Sciences, Medical College, Soochow University, Suzhou, Jiangsu 215123, PR China;1. Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, ROC;2. Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, ROC |
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| Abstract: | Taxifolin (TAX) is a natural source of bioflavonoid found in various conifers. In this study, initially we investigated the antioxidant potential of TAX under in vitro assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), ferric-ion reducing power (FRAP) and hydroxyl radical (OH). The activities of DPPH, ABTS, FRAP and OH radical levels were significantly inhibited by TAX with an IC50 values of 16.48, 66.34, 18.17 and 11.42 μg/ml, respectively. Secondly, TAX exhibited a strong protection against OH mediated DNA damage on pUC19 plasmid DNA at 1.0 μg/ml. Finally, we evaluated the protective mechanism of TAX against cadmium intoxicated zebrafish embryos (Danio rerio). We found that embryos exposed to 100 μM Cd exhibited significantly reduced survival, delayed hatching and phenotypic abnormalities at 24, 48, 72 and 96 hours post fertilization (hpf). Similarly, Cd intoxicated embryos showed significantly increased cardiac function (131 beats/min) at 60 hpf. Conversely, treatment with TAX (0.1, 1.0 and 10 μM) significantly enhanced the antioxidant enzyme levels (SOD, CAT, GPx and GR) by reducing the lipid peroxidation (MDA) in zebrafish embryos. Collectively, our results concluded that TAX could act as a potent redox scavenger against oxidative DNA damage and also functions as a crucial suppressor of Cd toxicity in zebrafish embryos. |
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| Keywords: | Taxifolin Antioxidants DNA damage protection Zebrafish embryo Cadmium Oxidative stress |
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