Effect of melamine on [Ca2+]i and viability in PC3 human prostate cancer cells |
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| Affiliation: | 1. School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan;2. Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan;3. Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;4. School of Public Health, College of Public Health, Taipei Medical University, Taipei, Taiwan;5. Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;6. Institute of Biomedical Engineering & Nanomedicine, National Health Research Institutes, Miaoli, Taiwan;7. Department of Pathology, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan;8. Institute of Occupational Safety and Health, Council of Labor Affairs, Executive Yuan, New Taipei City, Taiwan;9. School of Public Health, National Defense Medical Center, Taipei, Taiwan;10. Graduate Institute of Environmental Engineering, National Central University, Taoyuan, Taiwan;11. Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan;12. Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Taiwan;1. Department of Public Health, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan;2. Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;3. Department of Urology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;4. Pingtung Hospital, Department of Health, Executive Yuan, Pingtung, Taiwan;5. Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan;6. Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;7. Department of Family Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;8. Center of Environmental and Occupational Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan |
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| Abstract: | Melamine is thought to be an endocrine disrupter that affects physiology in cells. This study examined the effect of melamine on cytosolic free Ca2+ concentrations ([Ca2+]i) and viability in PC3 human prostate cancer cells. Melamine evoked [Ca2+]i rises concentration-dependently. Melamine-evoked Ca2+ entry was inhibited by nifedipine, econazole, SKF96365, GF109203X and phorbol 12-myristate 13 acetate. In Ca2+-free medium, treatment with the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin inhibited melamine-evoked [Ca2+]i rise. Conversely, treatment with melamine abolished thapsigargin-evoked [Ca2+]i rise. Inhibition of phospholipase C with U73122 did not alter melamine-evoked [Ca2+]i rise. Melamine at 500–800 μM decreased cell viability, which was not reversed by pretreatment with the Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid-acetoxymethyl ester (BAPTA/AM). Collectively, our data suggest that in PC3 cells, melamine induced [Ca2+]i rises by evoking phospholipase C-independent Ca2+ release from the endoplasmic reticulum, and Ca2+ entry via protein kinase C-regulated store-operated Ca2+ entry. Melamine also caused Ca2+-independent cell death. |
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| Keywords: | Human prostate cancer cells Melamine Cell death |
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