| E-Cadherin在修复性牙本质形成中的免疫组化研究 |
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| 引用本文： | 陈智,樊明文,边专,吕平,张旗. E-Cadherin在修复性牙本质形成中的免疫组化研究[J]. 牙体牙髓牙周病学杂志, 2000, 10(5): 268-270 |
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| 作者姓名： | 陈智 樊明文 边专 吕平 张旗 |
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| 作者单位： | 湖北医科大学口腔医学院，湖北 武汉 430079 |
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| 基金项目： | 湖北省自然科学基金资助课题(98J096) |
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| 摘 要： | 目的：观察细胞粘附分子Ｅ－ｃａｄｈｅｒｉｎ在修复性牙本质表成中的免疫反应和定位。方法：在大鼠上、下颌第一磨牙近中面制备单面洞，分别观察３、１５和３０ｄ。标本行常规组织学处理。利用鼠抗Ｅ－ｃａｄｈｅｒｉｎ为免疫组化ＳＡＢＣ法，对标本进行标记，ＨＥ复染。结果：Ｅ－ｃａｄｈｅｒｉｎ在术后３ｄ成牙本质细胞梁色较弱，牙髓细胞为阳性；１５ｄ后，成牙本质细胞样细胞和牙髓细胞染色阳性；３０ｄ后，成牙本质细胞样细胞
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| 关 键 词： | 细胞粘附分子 E－cadherin 修复性牙本质 |
| 文章编号： | 1005-2593(2000)05-0268-03 |
| 修稿时间： | 1999-11-15 |
Immunohistochemistry study of E - cadherin in reparative dentinogenesis |
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| CHEN Zhi,FAN Ming-wen,BIAN Zhuan,et al. Immunohistochemistry study of E - cadherin in reparative dentinogenesis[J]. Chinese Journal of Conservative Dentistry, 2000, 10(5): 268-270 |
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| Authors: | CHEN Zhi FAN Ming-wen BIAN Zhuan et al |
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| Abstract: | AIM: To investigate the immunological reactivity of E-cadherin in the reparative dentinogenesis. METHODS: The cavity was prepared on the mesial surface of the first molar of Wistar rats. After the periods of 3, 15, 30 days of post-operation, animal were sacrificed and paraffin sections were prepared through histological process. SABC method was used to detect the reactivity using rat anti-E-cadherin antibody. RESULTS: The reactivity was not evident in odontoblasts of 3 days group, but was evident in dental pulp cells; the positive staining was observed in odontoblast-like cells and pulp cells after 15 days; in 30 days, the polarized odontoblast-like cells were positively stained. CONCLUSION: E-cadherin may involve in the differentiation, and polarization of dental pulp cells. [ |
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| Keywords: | cell adhesion Molecule E-cadherin reparative dentin odontoblast |
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