| 内皮抑素抑制人脐静脉内皮细胞分泌IL—6和IL—8的实验研究 |
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| 摘 要： |
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| 关 键 词： | 内皮抑素 内皮细胞 白细胞介素－6 白细胞介素－8 |
Inhibitory effects of endostatin on interleukin-6 and interleukin-8 release by human umbilical vein endothelial cells] |
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| Lin Zhang,Li-Min Luo,Hu Xia,Lie-Qian Zhuang,Wan-Cheng Tong,Jin-Xu Wen. Inhibitory effects of endostatin on interleukin-6 and interleukin-8 release by human umbilical vein endothelial cells][J]. Journal of First Military Medical University, 2002, 22(1): 54-56 |
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| Authors: | Lin Zhang Li-Min Luo Hu Xia Lie-Qian Zhuang Wan-Cheng Tong Jin-Xu Wen |
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| Affiliation: | Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. |
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| Abstract: | OBJECTIVE: To investigate the inhibitory effects exercised by endostatin on the production of interleukin-6 (IL-6) and IL-8 by human umbilical vein endothelial cells (HUVECs). METHODS:(HUVECs were isolated and cultured in vitro with endostatin (treated group) or PBS (control group), and the supernatant was harvested from the primary culture medium daily for 9 consecutive days starting from the first day of culture, followed by centrifugation. IL-6 and IL-8 contents in the supernatant were measured using sandwich enzyme-linked immunosorbent assay (ELISA). RESULTS: IL-6 and IL-8 were detected in the supernatant of the control cell culture, and their amounts increased as the cell culture was prolonged, reaching the peak levels on day 6 (2 979.32+/-19.65 pg/ml and 6 018.87+/-56.74 pg/ml, respectively). In the treated group, however, the amounts of IL-6 and IL-8 were significantly lower than the control levels (P<0.01). CONCLUSION: Endostatin can inhibit the growth and proliferation of endothelial cells, reducing their biological activities. |
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