| ISSR法鉴定中药玉竹与小玉竹 |
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| 引用本文： | 周晔,唐铖,安适之,张攻,王润玲.ISSR法鉴定中药玉竹与小玉竹[J].中医药学报,2006,34(5):7-9. |
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| 作者姓名： | 周晔 唐铖 安适之 张攻 王润玲 |
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| 作者单位： | 天津医科大学药学院,天津,300070 |
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| 基金项目： | 天津市卫生局资助项目;天津医科大学校科研和教改项目 |
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| 摘 要： | 目的:鉴别中药玉竹及其掺伪品小玉竹,探索中药玉竹鉴别的新方法。方法:采用显微鉴定和ISSR法鉴定中药玉竹,共考察了28条随机引物,从中筛选出引物(CA)8G,(ATG)5用于分析,经18%琼脂糖电泳分离产物。结果:所选引物进行的PCR扩增可产生用于分析的多态性谱带。通过显微鉴定以及ISSR标记法可以将玉竹及其主要掺伪品区分开。结论:本实验所建立的ISSR标记方法可用于中药玉竹和小玉竹的区分。
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| 关 键 词： | 玉竹 小玉竹 显微鉴定 |
| 文章编号： | 1002-2392（2006）05-0007-03 |
| 收稿时间： | 2006-04-30 |
| 修稿时间： | 2006年4月30日 |
Identification of Polygonatum odoratum and P. Humile by ISSR |
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| ZHOU Ye,TANG Cheng,AN Shi-zhi,et alAuthor's address:College of Pharmacy Tianjin Medical University,Tianjin ,Chin.Identification of Polygonatum odoratum and P. Humile by ISSR[J].Acta Chinese Medicine and Pharmacology,2006,34(5):7-9. |
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| Authors: | ZHOU Ye TANG Cheng AN Shi-zhi Author's address:College of Pharmacy Tianjin Medical University Tianjin Chin |
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| Institution: | ZHOU Ye,TANG Cheng,AN Shi-zhi,et alAuthor's address:College of Pharmacy Tianjin Medical University,Tianjin 300070,Chin |
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| Abstract: | Objective:To distinguish Polygonatum odroatum from tis counterfeit P.humile and set up a new method to identify the former.Methods:Microscopic and ISSR method were used for the identification of Polygonatum odoratum.28 arbitrary primers were evaluated,and two of them such as(CA)_8G,(ATG)_5 were selected.All the samples were separated by 1.8% gelose electrophoresis.Results:The selected primers could yield enough polymorphic bands for the analysis.With the ISSR and microscopic method,Polygonatum odoratum and its counterfeit P.humile could be identified.Conclusion:The ISSR method that was set up in this research is useful for the identification of Polygonatum odoratum and its Counterfeit P.humile. |
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| Keywords: | ISSR |
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