| Liposome-mediated Functional Expression of Multiple Drug Resistance Gene in Human Bone Marrow CD34＋ Cells |
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| 引用本文： | 曹文静,邹萍.Liposome-mediated Functional Expression of Multiple Drug Resistance Gene in Human Bone Marrow CD34＋ Cells[J].华中科技大学学报(医学英德文版),2004,24(3):214-215,235. |
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| 作者姓名： | 曹文静 邹萍 |
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| 作者单位： | Department of Laboratory Medicine，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Institute of Hematology，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022，China |
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| 摘 要： | Myelosuppressionisanimportantdose limitingfactorformostofchemotherapeuticagentsinclinicalpractice.OverexpressionofP glycoprotein (P gp)encodedbyMDR1geneisaccompaniedbyfunctionaldrugresistance.TheexpressionofP gpisconsistent lylowinnormalbonemarrowcells,an…
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| 关 键 词： | 脂肪体介导 功能表达 麻药抵抗基因 骨髓CD34＋细胞 基因转染 |
Liposome-mediated Functional Expression of Multiple Drug Resistance Gene in Human Bone Marrow CD34+ Cells |
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| CAO Wenjing,ZOU Ping.Liposome-mediated Functional Expression of Multiple Drug Resistance Gene in Human Bone Marrow CD34+ Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2004,24(3):214-215,235. |
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| Authors: | CAO Wenjing ZOU Ping |
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| Institution: | 1. Department of Laboratory Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
2. Institute of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China |
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| Abstract: | The expression and functional activity of multiple drug resistance (MDR1) gene in human normal bone marrow CD34+ cells was observed. Human normal bone marrow CD34+ cells were enriched with magnetic cell sorting (MACS) system, and then liposome-mediated MDR1 gene was transferred into bone marrow CD34+ cells. Fluorescence-activated cell sorter was used to evaluate the expression and functional activity of P-glycoprotein (P-gp) encoded by MDR1 gene. It was found that the purity of bone marrow CD34+ cells was approximately (91 +/- 4.56)% and recovery rate was (72.3 +/- 2.36)% by MACS. The expression of P-gp in the transfected CD34+ cells was obviously higher than that in non-transfected CD34+ cells. The amount of P-gp in non-transfected CD34+ cells was (11.2 +/- 2.2)%, but increased to (23.6 +/- 2.34)% 48 h after gene transfection (P<0.01). The amount of P-gp was gradually decreased to the basic level one week later. The accumulation and extrusion assays showed that the overexpression of P-gp could efflux Rh-123 out of cells and there was low fluorescence within the transfected cells. The functional activity of P-gp could be inhibited by 10 microg/ml verapamil. It was suggested that the transient and highly effective expression and functional activity of P-gp could be obtained by liposome-mediated MRD1 transferring into human normal bone marrow CD34+ cells. |
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| Keywords: | gene transfection hematopoietic progenitor cell multiple drug resistance gene P-glycoprotein |
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