流式细胞术与PCR-SSP法检测HLA-B27的比对及HLA-B27亚型的研究

目的探讨流式细胞术(FCM)与序列特异性引物聚合酶链式反应(PCR-SSP)在人类白细胞表面抗原-B27(HLA-B27)检测中的应用,并观察HLA-B27亚型在本地区的分布情况。方法采用PCR-SSP和FCM检测88例疑似强直性脊柱炎(AS)患者HLA-B27。结果 88例AS疑似患者中FCM法检测HLA-B27结果阳性82例,阴性6例,阳性率为93.2%;PCR-SSP法检测阳性83例、阴性5例、阳性率为94.3%。分型结果 B*2705型41例占49.4%;B*2704型37例占44.5%;B*2702型1例占1.2%;阳性但未分辨型别4例。结论 Kappa一致性系数K=0.905,PCR-SSP法与FCM检测HLA-B27结果具有较高的一致性。本地区易感HLA-B27等位基因亚型主要以HLA-B*2704和HLA-B*2705为主。

Comparison of HLA-B27 detection by flow cytometry and SSP-PCR and study on HLA-B27 subtypes

Objective To investigate the application of flow cytometry (FCM ) and sequence‐specific primer polymerase chain reaction (PCR‐SSP) method in the detection of human leukocyte antigen‐B27 (HLA‐B27) and to observe the distribution of HLA‐B27 subtypes in the local region .Methods The HLA‐B27 level in 88 cases of sus‐pected ankylosing spondylitis (AS) was detected by FCM and PCR‐SSP .Results Among 88 cases of suspected AS , 82 cases were HLA‐B27 positive and 6 cases were HLA‐B27 negative by FCM ,the positive rate was 93 .2% ;whereas 83 cases were HLA‐B27 positive and 5 cases were HLA‐B27 negative by PCR‐SSP ,the positive rate was 94 .3% .In the subtyping results ,41 cases were B * 2705 genotype ,accounting for 49 .4% ,37 cases were B * 2704 genotype ,ac‐counting for 44 .5% ,1 case was B * 2702 genotype ,accounting for 1 .2% ,4 cases were positive without distinguished genotype .Conclusion The Kappa consistency coefficient is 0 .905 ,the detection results of HLA‐B27 by PCR‐SSP and FCM have higher consistency .The susceptible HLA‐B27 allele subtypes are mainly HLA‐B * 2704 and HLA‐B *2705 .