惠州地区高危型HPVE6/E7 mRNA检测在宫颈癌筛查中的临床意义

目的探讨与液基细胞学(TCT)检测结果比较,分析高危型人乳头瘤病毒(HPV)E6/E7 mRNA检测在宫颈癌筛查中的临床价值。方法选取该院妇科接受TCT检查的140例患者,根据检查结果分为5个组:炎性/良性组(32例),宫颈上皮内瘤变(CIN)Ⅰ组(44例),INⅡ组(38例),CINⅢ组(17例),宫颈癌组(9例)。比较各组HPV E6/E7mRNA检测阳性率和表达水平。结果 CIN异型程度增高,HPV E6/E7 mRNA阳性率和表达水平均呈增加趋势,不同CIN分级患者的阳性率和表达水平比较,差异均有统计学意义(P0.05);与TCT病理检查结果有较高的一致性。结论高危型HPV E6/E7 mRNA可以作为惠州地区宫颈癌筛查手段之一,联合HPV E6/E7 mRNA及TCT检测可提高其诊断准确性。

Clinical significance of detection of high-risk type HPV E6/E7 mRNA in cervical cancer screening in Huizhou

Objective Comparing with the liquid based cytology test results ,we analyzed the performance of high‐risk human papilloma virus E6/E7 mRNA detection in cervical cancer diagnosis ,and discussed the role and sig‐nificance in cervical cancer screening in Huizhou .Methods Liquid based cytology specimens of 140 patients who ac‐cepted the liquid based cytology test were selected in Gynecology department of our hospital .According to patients outcomes ,they were divided into 4 groups :inflammation/benign group (32 cases) ,cervical intraepithelial neoplasia (CIN) I group (44 cases) ,CIN II Group (38 cases) ,CIN Ⅲ Group (17 cases) and cervical carcinoma group (9 ca‐ses) .We compared the positive rate of HPV E6/E7 mRNA detection and expression level in different groups by using t test for statistical analysis .Results With the increase of CIN heterotype ,HPV E6/E7 mRNA positive rate and ex‐pression level showed a trend of increase .In patients with different grades of CIN ,the differences of positive rate and expression level of HPV E6/E7 mRNA detection were statistically significant (P< 0 .05) ,and the results were con‐sistent with the results of TCT pathology .Conclusion High‐risk type HPV E6/E7 mRNA detection can be used as one of cervical cancer screening in Huizhou ,and the combination of HPV E6/E7 mRNA and cytological examination can improve the diagnostic accuracy of cervical cancer .