人旋毛虫病血清与诊断抗原的检测方法的比较研究

以旋毛虫肌肉期幼虫的分泌排泄抗原（ES）和纯化ES中46～58KD抗原（P－ES）为诊断抗原，用Dot－ELISA同时检测34份旋毛虫病人血清，阳性检出率分别为91．18％和94．12％（P＞0．05）．而100份正常人血清均为阴性；检测其它4种蠕虫病人血清62份，交叉反应率分别为29．3％和0％，P－ES的特异性明显优于ES（P＜0．05）。另外，以P-ES为珍断抗原，比较Dot-ELISA和间接ELISA用于诊断旋毛虫病人的检测效果，实验表明：Dot-ELISA不仅有间接ELISA相似的敏感性和特异性，而且简便、快速、经济，更适合于推广应用。

COMPARATIVE STUDIES ON SERODIAGNOSTIC METHODS AND DIAGNOSTIC ANTIGENS FOR HUMAN TRICHINOSIS

In the first place, the diagnostic value of excretorysecretory antigen (ES) of Trichinella spiralis muscle larvae and its purified 46-58 KD antigen (P-ES) was tested by Dot-ELISA. The positive rates of 34 sera from confirmed trichinosis patients using ES and P-ES as diagnostic antigen were 91. 18% and 94. 12% respectively (P>0. 05). The false positive reactions of 100 normal human sera were not found. in the study. The cross reaction rates of 62 sera of patients with other helminthiasis were 29. 03% and 0% respectively, the specifity of P-ES was obviouly higher than that of ES (P<0. 05) Next, comparision of Dot-ELISA and indirect ELISA using P-ES as diagnostic antigen for detecting human trichinosis was made. The results indicated that Dot-ELISA is not only as sensitive and specific as indirect ELISA but more rapid, simple, economical and feasible in field work or in rural area.