几丁糖对不同来源成纤维细胞生物学特性的影响

目的　探讨医用几丁糖 (以下简称几丁糖 )对病理性瘢痕成纤维细胞生物学活性的作用。方法　以瘢痕疙瘩及增生性瘢痕成纤维细胞为研究对象 ,正常皮肤成纤维细胞为对照 ,用组织块法进行不同标本成纤维细胞体外培养。分别观测不同浓度的几丁糖作用后 ,不同来源成纤维细胞吸光度A、形态结构、合成及分泌胶原的量以及细胞因子TGF β1 、b FGF、IL 8等的变化。结果　几丁糖对不同来源成纤维细胞增殖及胶原、TGF β1 、b FGF、等的分泌均呈剂量依赖性抑制 ,对IL 8则促进 ;且对各组的影响差异无显著性意义。结论　几丁糖可以抑制瘢痕疙瘩和增生性瘢痕成纤维细胞的生长、增殖、合成及分泌胶原功能 ,有望在病理性瘢痕的防治中发挥重要作用。

The effects of chitosan on different-derived fibroblasts

OBJECTIVE: To investigate the effects of chitosan on the biological activities of the fibroblasts derived from different tissues. METHODS: The biological activities of the fibroblasts derived from different tissues were evaluated with a MTT method for fibroblast proliferation, photic and electronic microscope for morphologic and subcellular structure, 3H-proline uptake method for collagen secretion and ELISA box for the secretion of TGF-beta 1, FGF-AB, and IL-8. RESULTS: This study showed that the chitosan inhabited the proliferation of the fibroblasts and the secretion of the TGF-beta 1, FGF-AB and collagen of the fibroblasts with a dose-depended manner in the normal skin, hypertrophic scar and keloid groups, but it stimulated the IL-8. However, there were no significant differences among the three groups (P > 0.05). CONCLUSION: The chitosan could inhibit the growth, proliferation, biosynthesis and secretion of the fibroblasts, and it may be used to treat different scars.