消风宣窍汤对TDI致过敏性鼻炎模型豚鼠血清IL-5、IL-12表达水平的影响

目的:观察消风宣窍汤对过敏性鼻炎豚鼠行为学及细胞因子的影响,以探讨其治疗机制。方法:40只Hartley豚鼠随机分为正常组、模型组、消风宣窍汤组、氯雷他定组,每组10只。采用10%二异氰酸甲苯酯(TDI)橄榄油溶液致敏豚鼠建立过敏性鼻炎动物模型,灌胃治疗1周,观察豚鼠行为学变化,用ELISA法测定血清中细胞因子IL-5、IL-12含量。结果:消风宣窍汤能明显减轻豚鼠过敏性鼻炎鼻部过敏症状以及鼻黏膜上皮炎细胞的浸润,对过敏性鼻炎豚鼠血清IL-5有显著的降低作用(P0.01),对IL-12有显著的升高作用(P0.01),两者呈负相关关系,并且效果优于开瑞坦组(P0.05)。结论:消风宣窍汤治疗AR的机制可能是通过降低IL-5和升高IL-12水平实现的,提示IL-5和IL-12在AR发病机制中有重要作用。

Effect of Xiaofeng Xuanqiao Decoction on Expression of Serum IL-5 and IL-12 in Allergic Rhinitis Models Induced by TDI

To study the influence of Xiaofeng Xuanqiao decoction on behavior and cytokines in allergic rhinitis models,and explore the therapeutic mechanism.Methods：Forty guinea pigs were randomized into 4 groups,normal control group,allergic rhinitis （AR）group,Xiaofeng Xuanqiao decoction group,and loratadine group.Allergic rhinitis models of guinea pigs were made by 1 0%TDI.Xiaofeng Xuanqiao decoction and loratadine were applied to inAR guinea pigs.The serum levels of IL-5 and IL-1 2 were determined by ELISA methods.Results：Xiaofeng Xuanqiao decoction could obviously relieve the hypersensitivity and infiltrate the inflammatory cells on the nasal mucosa.The serum level of IL-5 in Xiaofeng Xuanqiao decoction group significantly decreased compared with that of AR group（P〈0.01 ）,and the serum level of IL-1 2 in Xiaofeng Xuanqiao decoction group significantly increased （P〈0.01 ）.There is a positive correlation between them.The therapeutic effect of Xiaofeng Xuanqiao decoction group was better than that of loratadine group.Conclusion：The therapeutic mechanism of Xiaofeng Xuanqiao decoction on allergic rhinitis is decreasing the level of IL-5 and increasing the level of IL-1 2,which suggests that IL-5 and IL-1 2 are important factors in the pathogenesis of AR.