隔药饼灸对急性胃黏膜损伤家兔腧穴局部能量代谢的影响

目的:观察隔药饼灸对急性胃黏膜损伤家兔黏液层阳性层厚度和腧穴局部组织Na+/K+-ATP酶活性的影响,探讨隔药饼灸治疗急性胃黏膜损伤的机制。方法:采用无水乙醇损伤造模法建立急性胃黏膜损伤模型,将40只家兔随机分成正常组和造模组,待模型在确定后,造模组家兔再随机分成模型组、隔药饼灸组和西药组,每组9只。隔药饼灸组将制好的药饼置于后三里穴上,灸6壮,1次/d,连续灸7 d。其他各组仅做与隔药灸组相同的固定,不予任何治疗,共7 d。干预后,收集胃黏膜组织和后三里穴组织,分别按照实验处理要求做相应的处理。结果:隔药饼灸组胃黏膜黏液层阳性厚度较模型组明显增厚(P0.05),隔药饼灸组后三里穴的Na+/K+-ATP酶活性较模型组显著升高。结论:改善"后三里"腧穴能量代谢可能是保护和修复胃黏膜损伤的作用途径之一。

Effects of Herbs-Partitioned moxibustion on energy metabolism of local acupoint in rabbits with acute gastric mucosal lesion

Objective：To observe the effects of Herbs-Partitioned moxibustion on the positive mucus layer thickness and Na ＋/K＋-ATP enzymatic activity in rabbits with acute gastric mucosal lesion for exploring the mechanisms of moxibustion action on model of acute gas -tric mucosal lesion.Methods：Anhydrous ethanol damage is adopted to establish model of gastric mucosal lesion.30 of 40 rabbits were randomly selected as model group ,the rest as control.After the successful models were confirmed ,30 model rabbits were randomly alloca-ted to model group , herbs-partition moxibustion group and western medicine group.Herbs partition moxibustion group were treated by moxibustion on Housanli points ,6 columns once a day.Western medicine group were administrated by gavage at dosage of 25 mg/kg a day.The other groups just fixed the same as herbs-partition moxibustion group without any treatment.The treatments continued for 7 days.the gastric mucosa and tissues of Housanli points were collected on 8th day,corresponding procedures were done at request of ex-periment.Results：The positive mucus layer thickness of herbs-partition moxibustion group was more significantly increased than those of model group （ P〈0.05 ）.The Na ＋/K＋-ATP enzymatic activity of herbs-partition moxibustion group was significantly increased than those of model group.Conclusion：Improving the local energy metabolism of local acupoint in rabbits with acute gastric mucosal lesion may be one of the mechanisms of restoring and protecting the mucosal lession .