| 肝细胞色素P450 2E1在大鼠非酒精性脂肪肝发生中的作用 |
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| 引用本文： | 胡辂,陈东风,史洪涛.肝细胞色素P450 2E1在大鼠非酒精性脂肪肝发生中的作用[J].第三军医大学学报,2004,26(17):1561-1563. |
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| 作者姓名： | 胡辂 陈东风 史洪涛 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所消化内科,重庆,400042;第三军医大学大坪医院野战外科研究所消化内科,重庆,400042;第三军医大学大坪医院野战外科研究所消化内科,重庆,400042 |
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| 摘 要： | 目的研究大鼠非酒精性脂肪肝肝细胞色素P450 2E1表达变化及其在脂质过氧化反应中的作用.方法Wistar大鼠40只,随机分为正常对照组和高脂饮食诱导脂肪肝组,用免疫组织化学和Western blot方法测定肝细胞色素P450 2E1表达变化,酶学测定丙二醛含量.结果脂肪肝大鼠丙二醛含量较正常对照组增高,以高脂饮食8、12周为甚(P＜0.05),肝细胞色素P450 2E1表达亦明显增强(P＜0.01).结论肝细胞色素P450 2E1表达变化与脂肪肝引起的肝脏损害以及脂质过氧化反应程度密切相关.
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| 关 键 词： | 脂肪肝 细胞色素P4502E1 免疫组织化学 |
| 文章编号： | 1000-5404(2004)17-1561-03 |
| 修稿时间： | 2004年3月30日 |
Roles of hepatocyte cytochrome P450 2E1 in nonalcoholic steatosis of rat liver |
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| HU Lu,CHEN Dong feng,SHI Hong tao.Roles of hepatocyte cytochrome P450 2E1 in nonalcoholic steatosis of rat liver[J].Acta Academiae Medicinae Militaris Tertiae,2004,26(17):1561-1563. |
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| Authors: | HU Lu CHEN Dong feng SHI Hong tao |
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| Abstract: | Objective To study the roles of hepatocyte cytochrome P450 2E1 in model of nonalcoholic steatosis in rats Methods A total of 40 Wistar rats were randomly divided into two groups: control group (C) and high fat diet induced fatty liver group (H) The expression of hepatocyte cytochrome P450 2E1 antigen in rat model of nonalcoholic steatosis was detected by immunohistochemical method and Western blotting Malondialdehyde (MDA) contents were also determined Results MDA contents and the expression of hepatocyte cytochrome P450 2E1 antigen in rat model of nonalcoholic steatosis induced by high fat diet were higher than those in the normal controls ( P <0 05, P <0 01), and positively associated with the degrees of severity of nonalcoholic steatosis Conclusion Cytochrome P450 2E1 may be associated with liver damage due to nonalcoholic steatosis and lipid peroxidation |
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| Keywords: | fatty liver cytochrome P450 2E1 immunohisochemistry |
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