FISH检测HER-2基因在宫颈腺癌细胞中的表达及临床意义

目的:探讨HER-2基因在不同程度子宫颈病变脱落细胞中的表达以及临床意义。方法:采用荧光原位杂交技术(FISH)检测脱落细胞HER-2基因,其中宫颈腺癌(SCC)患者18例,上皮细胞内瘤变(CN)患者72例,正常细胞学妇女18例。结果:HER-2基因在正常对照、CNⅠ、CNⅡ/Ⅲ以及SCC患者中的阳性表达率分别为5.6%、10.2%、30.4%和50.0%。CNⅡ/Ⅲ、SCC患者与正常对照,HER-2基因阳性表达均有显著差别(P<0.05);SCC组与CNⅠ和CNⅡ/Ⅲ组比较,HER-2基因阳性表达差别也有统计学意义(P<0.05)。结论:HER-2基因高表达在子宫颈癌的发生、发展中可能起重要作用,FISH检测HER-2基因可以作为早期子宫颈癌筛查的方法之一。

Expression and clinical significance of HER-2 gene in cervical scraping samples

Objective:To investigate the expression and clinical significance of the HER-2 gene in cervicalscraping samples.Methods:The fluorescent in situ hybridization(FISH) was used to detect the expression of HER-2 gene in cervical scraping samples from squamous carcinomas of the cervix(SCC,n=18),cervical intraepithelial neoplasia(CN.n = 72) and normal control cervix(n = 18).Results:Positive expression rates of the HER-2 gene in normal control cervix,CNⅠ,CNⅡ/Ⅲ and SCC were 5.6%,10.2%,30.4%,50.0%.Compared with normal control cervix,HER-2 gene positive expression in CN Ⅱ/Ⅲ and SCC all had significant difference(P<0.05);There were also significant difference between SCC and CNⅠ,SCC and CNⅡ/Ⅲ(P<0.05).Conclusion:The over expression of HER-2 gene may play an important role in cervical carcinogenesis,therefore,it can be used as a better tumor index for early cervical cancer screening and accessory diagnosis.