T淋巴细胞疫苗诱导大鼠移植心存活时间延长的机制探讨

目的 探讨Ｔ淋巴细胞疫苗（ＴＣＶ）对大鼠移植心脏存活时间的影响及其机理。方法 取经供者抗原致敏的受者脾细胞（１／３脾切除）制备ＴＣＶ，然后将其回注于自身腹腔，观察大鼠异位移植心脏的存活时间。结果 接种ＴＣＶ可明显延长大鼠同种移植心的存活时间；注射ＴＣＶ后，Ｔ淋巴细胞增殖反应增强，Ｂ淋巴细胞增殖反应未受影响；混合淋巴细胞反应（ＭＬＲ）降低；脾细胞及ＴＣＶ表型分析显示ＣＤ８＾＋细胞增多；抗体介导的补体

Study on the mechanisms of T cell vaccination-induced survival prolongation of cardiac allograft in rats

Objective To investigate the effect of T cell vaccination (TCV) on the survival time of cardiac allograft in rat. Methods Rat heterotopic cardiac transplantation was performed. Donor antigen-primed recipients' spleen cells were obtained by 1/3 splenectomy for the preparation of TCV. TCV was then intraperitoneally into the abdominal cavity again. The survival time of cardiac allograft was observed. Results TCV could markedly prolonged rat cardiac allograft survival. T cell proliferation was increased and B cell proliferation was not affected in the vaccinated rats. The mixed lymphocyte reaction (MLR) was inhibited. The analysis of phenotypes on T cells showed that the number of CD8 clones in vaccine cells and vaccinated rat spleen cells were increased. The antibody-mediated cytotoxicity was not affected by TCV. Conclusions TCV enhances the recipients' T cell-mediated immune response. The TCV-induced hyporeactivity to specific donor antigen is related to the induction of anti-idiotypic response and is not associated with humoral mechanisms.